Gestational diabetes and the human salivary microbiota: a longitudinal study during pregnancy and postpartum
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Gestational diabetes and the human salivary microbiota : a longitudinal study during pregnancy and postpartum. / Crusell, Mie K.W.; Brink, Lærke R.; Nielsen, Trine; Allin, Kristine H.; Hansen, Torben; Damm, Peter; Lauenborg, Jeannet; Hansen, Tue H.; Pedersen, Oluf.
In: BMC Pregnancy and Childbirth, Vol. 20, 69, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Gestational diabetes and the human salivary microbiota
T2 - a longitudinal study during pregnancy and postpartum
AU - Crusell, Mie K.W.
AU - Brink, Lærke R.
AU - Nielsen, Trine
AU - Allin, Kristine H.
AU - Hansen, Torben
AU - Damm, Peter
AU - Lauenborg, Jeannet
AU - Hansen, Tue H.
AU - Pedersen, Oluf
PY - 2020
Y1 - 2020
N2 - Background: An aberrant composition of the salivary microbiota has been found in individuals with type 2 diabetes, and in pregnant women salivary microbiota composition has been associated with preeclampsia and pre-term birth. Pregnant women, who develop gestational diabetes (GDM), have a high risk of developing type 2 diabetes after pregnancy. In the present study we assessed whether GDM is linked to variation in the oral microbial community by examining the diversity and composition of the salivary microbiota. Method: In this observational study the salivary microbiota of pregnant women with GDM (n = 50) and normal glucose regulation (n = 160) in third trimester and 9 months postpartum was assessed by 16S rRNA gene amplicon sequencing of the V1-V3 region. GDM was diagnosed in accordance with the International Association of the Diabetes and Pregnancy Study Groups (IADPSG) criteria. Cross-sectional difference in alpha diversity was assessed using Student's t-test and longitudinal changes were assessed by mixed linear regression. Cross-sectional and longitudinal difference in beta diversity was assessed by permutational multivariate analyses of variance. Differentially abundant genera and OTUs were identified by negative binomial regression. Results: In the third trimester, two species-level operational taxonomic units (OTUs), while eight OTUs postpartum were differentially abundant in women with GDM compared with normoglycaemic women. OTU richness, Shannon diversity and Pielou evenness decreased from late pregnancy to 9 months after delivery regardless of glycaemic status. Conclusion: GDM is associated with a minor aberration of the salivary microbiota during late pregnancy and postpartum. For unknown reasons richness of the salivary microbiota decreased from late pregnancy to postpartum, which might be explained by the physiological changes of the immune system during human pregnancy.
AB - Background: An aberrant composition of the salivary microbiota has been found in individuals with type 2 diabetes, and in pregnant women salivary microbiota composition has been associated with preeclampsia and pre-term birth. Pregnant women, who develop gestational diabetes (GDM), have a high risk of developing type 2 diabetes after pregnancy. In the present study we assessed whether GDM is linked to variation in the oral microbial community by examining the diversity and composition of the salivary microbiota. Method: In this observational study the salivary microbiota of pregnant women with GDM (n = 50) and normal glucose regulation (n = 160) in third trimester and 9 months postpartum was assessed by 16S rRNA gene amplicon sequencing of the V1-V3 region. GDM was diagnosed in accordance with the International Association of the Diabetes and Pregnancy Study Groups (IADPSG) criteria. Cross-sectional difference in alpha diversity was assessed using Student's t-test and longitudinal changes were assessed by mixed linear regression. Cross-sectional and longitudinal difference in beta diversity was assessed by permutational multivariate analyses of variance. Differentially abundant genera and OTUs were identified by negative binomial regression. Results: In the third trimester, two species-level operational taxonomic units (OTUs), while eight OTUs postpartum were differentially abundant in women with GDM compared with normoglycaemic women. OTU richness, Shannon diversity and Pielou evenness decreased from late pregnancy to 9 months after delivery regardless of glycaemic status. Conclusion: GDM is associated with a minor aberration of the salivary microbiota during late pregnancy and postpartum. For unknown reasons richness of the salivary microbiota decreased from late pregnancy to postpartum, which might be explained by the physiological changes of the immune system during human pregnancy.
KW - Bacterial species
KW - Gestational diabetes mellitus
KW - Gestational hyperglycaemia
KW - Glycaemic traits
KW - Pregnancy
KW - Salivary microbiota
U2 - 10.1186/s12884-020-2764-y
DO - 10.1186/s12884-020-2764-y
M3 - Journal article
C2 - 32005194
AN - SCOPUS:85078861757
VL - 20
JO - B M C Pregnancy and Childbirth
JF - B M C Pregnancy and Childbirth
SN - 1471-2393
M1 - 69
ER -
ID: 243475655