Genomic investigation of Staphylococcus aureus isolates from bulk tank milk and dairy cows with clinical mastitis
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Genomic investigation of Staphylococcus aureus isolates from bulk tank milk and dairy cows with clinical mastitis. / Ronco, Troels; Klaas, Ilka C.; Stegger, Marc; Svennesen, Line; Astrup, Lærke B.; Farre, Michael; Pedersen, Karl.
In: Veterinary Microbiology, Vol. 215, 2018, p. 35-42.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Genomic investigation of Staphylococcus aureus isolates from bulk tank milk and dairy cows with clinical mastitis
AU - Ronco, Troels
AU - Klaas, Ilka C.
AU - Stegger, Marc
AU - Svennesen, Line
AU - Astrup, Lærke B.
AU - Farre, Michael
AU - Pedersen, Karl
PY - 2018
Y1 - 2018
N2 - Staphylococcus aureus is one of the most common pathogens that cause mastitis in dairy cows. Various subtypes, virulence genes and mobile genetic elements have been associated with isolates from bulk tank milk and clinical mastitis. So far, no Danish cattle associated S. aureus isolates have been whole-genome sequenced and further analyzed. Thus, the main objective was to investigate the population structure and genomic content of isolates from bulk tank milk and clinical mastitis, using whole-genome sequencing. This may reveal the origin of strains that cause clinical mastitis. S. aureus isolates from bulk tank milk (n = 94) and clinical mastitis (n = 63) were collected from 91 and 24 different farms, respectively and whole-genome sequenced. The genomic content was analyzed and a phylogenetic tree based on single nucleotide polymorphisms was constructed. In general, the isolates from both bulk tank milk and clinical mastitis were of similar genetic background. This suggests that dairy cows are natural carriers of the S. aureus subtypes that cause clinical mastitis if the right conditions are present and that a broad range of subtypes cause mastitis. A phylogenetic cluster that mostly consisted of ST151 isolates carried three mobile genetic elements that were primarily found in this group. The prevalence of resistance genes was generally low. However, the first ST398 methicillin resistant S. aureus isolate from a Danish dairy cow with clinical mastitis was detected.
AB - Staphylococcus aureus is one of the most common pathogens that cause mastitis in dairy cows. Various subtypes, virulence genes and mobile genetic elements have been associated with isolates from bulk tank milk and clinical mastitis. So far, no Danish cattle associated S. aureus isolates have been whole-genome sequenced and further analyzed. Thus, the main objective was to investigate the population structure and genomic content of isolates from bulk tank milk and clinical mastitis, using whole-genome sequencing. This may reveal the origin of strains that cause clinical mastitis. S. aureus isolates from bulk tank milk (n = 94) and clinical mastitis (n = 63) were collected from 91 and 24 different farms, respectively and whole-genome sequenced. The genomic content was analyzed and a phylogenetic tree based on single nucleotide polymorphisms was constructed. In general, the isolates from both bulk tank milk and clinical mastitis were of similar genetic background. This suggests that dairy cows are natural carriers of the S. aureus subtypes that cause clinical mastitis if the right conditions are present and that a broad range of subtypes cause mastitis. A phylogenetic cluster that mostly consisted of ST151 isolates carried three mobile genetic elements that were primarily found in this group. The prevalence of resistance genes was generally low. However, the first ST398 methicillin resistant S. aureus isolate from a Danish dairy cow with clinical mastitis was detected.
KW - Bulk tank milk
KW - Clinical mastitis
KW - Genomic analysis
KW - S. aureus
KW - Virulence and resistance genes
U2 - 10.1016/j.vetmic.2018.01.003
DO - 10.1016/j.vetmic.2018.01.003
M3 - Journal article
C2 - 29426404
AN - SCOPUS:85041478253
VL - 215
SP - 35
EP - 42
JO - Veterinary Microbiology
JF - Veterinary Microbiology
SN - 0378-1135
ER -
ID: 231412476