Genomic analysis of European bovine Staphylococcus aureus from clinical versus subclinical mastitis
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Genomic analysis of European bovine Staphylococcus aureus from clinical versus subclinical mastitis. / Hoekstra, Jurriaan; Zomer, Aldert L.; Rutten, Victor P.M.G.; Benedictus, Lindert; Stegeman, Arjan; Spaninks, Mirlin P.; Bennedsgaard, Torben W.; Biggs, Andrew; De Vliegher, Sarne; Mateo, Demetrio Herrera; Huber-Schlenstedt, Reglindis; Katholm, Jørgen; Kovács, Péter; Krömker, Volker; Lequeux, Guillaume; Moroni, Paolo; Pinho, Luís; Smulski, Sebastian; Supré, Karlien; Swinkels, Jantijn M.; Holmes, Mark A.; Lam, Theo J.G.M.; Koop, Gerrit.
In: Scientific Reports, Vol. 10, No. 1, 18172, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Genomic analysis of European bovine Staphylococcus aureus from clinical versus subclinical mastitis
AU - Hoekstra, Jurriaan
AU - Zomer, Aldert L.
AU - Rutten, Victor P.M.G.
AU - Benedictus, Lindert
AU - Stegeman, Arjan
AU - Spaninks, Mirlin P.
AU - Bennedsgaard, Torben W.
AU - Biggs, Andrew
AU - De Vliegher, Sarne
AU - Mateo, Demetrio Herrera
AU - Huber-Schlenstedt, Reglindis
AU - Katholm, Jørgen
AU - Kovács, Péter
AU - Krömker, Volker
AU - Lequeux, Guillaume
AU - Moroni, Paolo
AU - Pinho, Luís
AU - Smulski, Sebastian
AU - Supré, Karlien
AU - Swinkels, Jantijn M.
AU - Holmes, Mark A.
AU - Lam, Theo J.G.M.
AU - Koop, Gerrit
PY - 2020
Y1 - 2020
N2 - Intramammary infections (IMI) with Staphylococcus aureus are a common cause of bovine mastitis and can result in both clinical (CM) or subclinical mastitis (SCM). Although bacterial isolates of S. aureus differ in their virulence potential it is largely unclear which bacterial virulence factors are responsible for increased clinical severity. We performed a genome wide association study and used a generalized linear mixed model to investigate the correlation between gene carriage, lineage and clinical outcome of IMI in a collection of S. aureus isolates from cattle with CM (n = 125) and SCM (n = 151) from 11 European countries. An additional aim was to describe the genetic variation of bovine S. aureus in Europa. The dominant lineages in our collection were clonal complex (CC) 151 (81/276, 29.3%), CC97 (54/276, 19.6%), CC479 (32/276, 11.6%) and CC398 (19/276, 6.9%). Virulence and antimicrobial resistance (AMR) gene carriage was highly associated with CC. Among a selection of nine virulence and AMR genes, CC151, CC479 and CC133 carried more virulence genes than other CCs, and CC398 was associated with AMR gene carriage. Whereas CC151, CC97 were widespread in Europe, CC479, CC398 and CC8 were only found in specific countries. Compared to CC151, CC479 was associated with CM rather than SCM (OR 3.62; 95% CI 1.38–9.50) and the other CCs were not. Multiple genes were associated with CM, but due to the clustering within CC of carriage of these genes, it was not possible to differentiate between the effect of gene carriage and CC on clinical outcome of IMI. Nevertheless, this study demonstrates that characterization of S. aureus CC and virulence genes helps to predict the likelihood of the occurrence of CM following S. aureus IMI and highlights the potential benefit of diagnostics tools to identify S. aureus CC during bovine mastitis.
AB - Intramammary infections (IMI) with Staphylococcus aureus are a common cause of bovine mastitis and can result in both clinical (CM) or subclinical mastitis (SCM). Although bacterial isolates of S. aureus differ in their virulence potential it is largely unclear which bacterial virulence factors are responsible for increased clinical severity. We performed a genome wide association study and used a generalized linear mixed model to investigate the correlation between gene carriage, lineage and clinical outcome of IMI in a collection of S. aureus isolates from cattle with CM (n = 125) and SCM (n = 151) from 11 European countries. An additional aim was to describe the genetic variation of bovine S. aureus in Europa. The dominant lineages in our collection were clonal complex (CC) 151 (81/276, 29.3%), CC97 (54/276, 19.6%), CC479 (32/276, 11.6%) and CC398 (19/276, 6.9%). Virulence and antimicrobial resistance (AMR) gene carriage was highly associated with CC. Among a selection of nine virulence and AMR genes, CC151, CC479 and CC133 carried more virulence genes than other CCs, and CC398 was associated with AMR gene carriage. Whereas CC151, CC97 were widespread in Europe, CC479, CC398 and CC8 were only found in specific countries. Compared to CC151, CC479 was associated with CM rather than SCM (OR 3.62; 95% CI 1.38–9.50) and the other CCs were not. Multiple genes were associated with CM, but due to the clustering within CC of carriage of these genes, it was not possible to differentiate between the effect of gene carriage and CC on clinical outcome of IMI. Nevertheless, this study demonstrates that characterization of S. aureus CC and virulence genes helps to predict the likelihood of the occurrence of CM following S. aureus IMI and highlights the potential benefit of diagnostics tools to identify S. aureus CC during bovine mastitis.
U2 - 10.1038/s41598-020-75179-2
DO - 10.1038/s41598-020-75179-2
M3 - Journal article
C2 - 33097797
AN - SCOPUS:85093927694
VL - 10
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 18172
ER -
ID: 250820904