Genetic Deletion of Hesx1 Promotes Exit from the Pluripotent State and Impairs Developmental Diapause

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Genetic Deletion of Hesx1 Promotes Exit from the Pluripotent State and Impairs Developmental Diapause. / Pozzi, Sara; Bowling, Sarah; Apps, John; Brickman, Joshua M; Rodriguez, Tristan A; Martinez-Barbera, Juan Pedro.

In: Stem Cell Reports, Vol. 13, No. 6, 10.12.2019, p. 970-979.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Pozzi, S, Bowling, S, Apps, J, Brickman, JM, Rodriguez, TA & Martinez-Barbera, JP 2019, 'Genetic Deletion of Hesx1 Promotes Exit from the Pluripotent State and Impairs Developmental Diapause', Stem Cell Reports, vol. 13, no. 6, pp. 970-979. https://doi.org/10.1016/j.stemcr.2019.10.014

APA

Pozzi, S., Bowling, S., Apps, J., Brickman, J. M., Rodriguez, T. A., & Martinez-Barbera, J. P. (2019). Genetic Deletion of Hesx1 Promotes Exit from the Pluripotent State and Impairs Developmental Diapause. Stem Cell Reports, 13(6), 970-979. https://doi.org/10.1016/j.stemcr.2019.10.014

Vancouver

Pozzi S, Bowling S, Apps J, Brickman JM, Rodriguez TA, Martinez-Barbera JP. Genetic Deletion of Hesx1 Promotes Exit from the Pluripotent State and Impairs Developmental Diapause. Stem Cell Reports. 2019 Dec 10;13(6):970-979. https://doi.org/10.1016/j.stemcr.2019.10.014

Author

Pozzi, Sara ; Bowling, Sarah ; Apps, John ; Brickman, Joshua M ; Rodriguez, Tristan A ; Martinez-Barbera, Juan Pedro. / Genetic Deletion of Hesx1 Promotes Exit from the Pluripotent State and Impairs Developmental Diapause. In: Stem Cell Reports. 2019 ; Vol. 13, No. 6. pp. 970-979.

Bibtex

@article{3baf0719c2a84bf2b1e1323b11654121,

title = "Genetic Deletion of Hesx1 Promotes Exit from the Pluripotent State and Impairs Developmental Diapause",

abstract = "The role of the homeobox transcriptional repressor HESX1 in embryonic stem cells (ESCs) remains mostly unknown. Here, we show that Hesx1 is expressed in the preimplantation mouse embryo, where it is required during developmental diapause. Absence of Hesx1 leads to reduced expression of epiblast and primitive endoderm determinants and failure of diapaused embryos to resume embryonic development after implantation. Genetic deletion of Hesx1 impairs self-renewal and promotes differentiation toward epiblast by reducing the expression of pluripotency factors and decreasing the activity of LIF/STAT3 signaling. We reveal that Hesx1-deficient ESCs show elevated ERK pathway activation, resulting in accelerated differentiation toward primitive endoderm, which can be prevented by overexpression of Hesx1. Together, our data provide evidence for a novel role of Hesx1 in the control of self-renewal and maintenance of the undifferentiated state in ESCs and mouse embryos.",

author = "Sara Pozzi and Sarah Bowling and John Apps and Brickman, {Joshua M} and Rodriguez, {Tristan A} and Martinez-Barbera, {Juan Pedro}",

year = "2019",

month = dec,

day = "10",

doi = "10.1016/j.stemcr.2019.10.014",

language = "English",

volume = "13",

pages = "970--979",

journal = "Stem Cell Reports",

issn = "2213-6711",

publisher = "Cell Press",

number = "6",

}

RIS

TY - JOUR

T1 - Genetic Deletion of Hesx1 Promotes Exit from the Pluripotent State and Impairs Developmental Diapause

AU - Pozzi, Sara

AU - Bowling, Sarah

AU - Apps, John

AU - Brickman, Joshua M

AU - Rodriguez, Tristan A

AU - Martinez-Barbera, Juan Pedro

PY - 2019/12/10

Y1 - 2019/12/10

N2 - The role of the homeobox transcriptional repressor HESX1 in embryonic stem cells (ESCs) remains mostly unknown. Here, we show that Hesx1 is expressed in the preimplantation mouse embryo, where it is required during developmental diapause. Absence of Hesx1 leads to reduced expression of epiblast and primitive endoderm determinants and failure of diapaused embryos to resume embryonic development after implantation. Genetic deletion of Hesx1 impairs self-renewal and promotes differentiation toward epiblast by reducing the expression of pluripotency factors and decreasing the activity of LIF/STAT3 signaling. We reveal that Hesx1-deficient ESCs show elevated ERK pathway activation, resulting in accelerated differentiation toward primitive endoderm, which can be prevented by overexpression of Hesx1. Together, our data provide evidence for a novel role of Hesx1 in the control of self-renewal and maintenance of the undifferentiated state in ESCs and mouse embryos.

AB - The role of the homeobox transcriptional repressor HESX1 in embryonic stem cells (ESCs) remains mostly unknown. Here, we show that Hesx1 is expressed in the preimplantation mouse embryo, where it is required during developmental diapause. Absence of Hesx1 leads to reduced expression of epiblast and primitive endoderm determinants and failure of diapaused embryos to resume embryonic development after implantation. Genetic deletion of Hesx1 impairs self-renewal and promotes differentiation toward epiblast by reducing the expression of pluripotency factors and decreasing the activity of LIF/STAT3 signaling. We reveal that Hesx1-deficient ESCs show elevated ERK pathway activation, resulting in accelerated differentiation toward primitive endoderm, which can be prevented by overexpression of Hesx1. Together, our data provide evidence for a novel role of Hesx1 in the control of self-renewal and maintenance of the undifferentiated state in ESCs and mouse embryos.

U2 - 10.1016/j.stemcr.2019.10.014

DO - 10.1016/j.stemcr.2019.10.014

M3 - Journal article

C2 - 31761678

VL - 13

SP - 970

EP - 979

JO - Stem Cell Reports

JF - Stem Cell Reports

SN - 2213-6711

IS - 6

ER -

ID: 235343715

Forskning