From pluripotency to totipotency: an experimentalist's guide to cellular potency
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From pluripotency to totipotency : an experimentalist's guide to cellular potency. / Riveiro, Alba Redo; Brickman, Joshua Mark.
In: Development, Vol. 147, No. 16, 189845, 08.2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - From pluripotency to totipotency
T2 - an experimentalist's guide to cellular potency
AU - Riveiro, Alba Redo
AU - Brickman, Joshua Mark
PY - 2020/8
Y1 - 2020/8
N2 - Embryonic stem cells (ESCs) are derived from the pre-implantation mammalian blastocyst. At this point in time, the newly formed embryo is concerned with the generation and expansion of both the embryonic lineages required to build the embryo and the extra-embryonic lineages that support development. When used in grafting experiments, embryonic cells from early developmental stages can contribute to both embryonic and extra-embryonic lineages, but it is generally accepted that ESCs can give rise to only embryonic lineages. As a result, they are referred to as pluripotent, rather than totipotent. Here, we consider the experimental potential of various ESC populations and a number of recently identified in vitro culture systems producing states beyond pluripotency and reminiscent of those observed during pre-implantation development. We also consider the nature of totipotency and the extent to which cell populations in these culture systems exhibit this property.
AB - Embryonic stem cells (ESCs) are derived from the pre-implantation mammalian blastocyst. At this point in time, the newly formed embryo is concerned with the generation and expansion of both the embryonic lineages required to build the embryo and the extra-embryonic lineages that support development. When used in grafting experiments, embryonic cells from early developmental stages can contribute to both embryonic and extra-embryonic lineages, but it is generally accepted that ESCs can give rise to only embryonic lineages. As a result, they are referred to as pluripotent, rather than totipotent. Here, we consider the experimental potential of various ESC populations and a number of recently identified in vitro culture systems producing states beyond pluripotency and reminiscent of those observed during pre-implantation development. We also consider the nature of totipotency and the extent to which cell populations in these culture systems exhibit this property.
KW - Developmental biology
KW - Pluripotency
KW - Totipotency
KW - EMBRYONIC STEM-CELLS
KW - BLASTOCYST-LIKE STRUCTURES
KW - PRIMITIVE ENDODERM
KW - MOUSE EMBRYOS
KW - GROUND-STATE
KW - SELF-RENEWAL
KW - LINEAGE SEGREGATION
KW - 2-CELL EMBRYOS
KW - FATE DECISIONS
KW - INNER
U2 - 10.1242/dev.189845
DO - 10.1242/dev.189845
M3 - Journal article
C2 - 32847824
VL - 147
JO - Development
JF - Development
SN - 0950-1991
IS - 16
M1 - 189845
ER -
ID: 256979418