Focused B cell response to recurring gluten motif with implications for epitope spreading in celiac disease

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Focused B cell response to recurring gluten motif with implications for epitope spreading in celiac disease. / Zhou, Chunyan; Østerbye, Thomas; Bach, Emil; Dahal-Koirala, Shiva; Høydahl, Lene S.; Steinsbø, Øyvind; Jahnsen, Jørgen; Lundin, Knut E.A.; Buus, Søren; Sollid, Ludvig M.; Iversen, Rasmus.

In: Cell Reports, Vol. 41, No. 4, 111541, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Zhou, C, Østerbye, T, Bach, E, Dahal-Koirala, S, Høydahl, LS, Steinsbø, Ø, Jahnsen, J, Lundin, KEA, Buus, S, Sollid, LM & Iversen, R 2022, 'Focused B cell response to recurring gluten motif with implications for epitope spreading in celiac disease', Cell Reports, vol. 41, no. 4, 111541. https://doi.org/10.1016/j.celrep.2022.111541

APA

Zhou, C., Østerbye, T., Bach, E., Dahal-Koirala, S., Høydahl, L. S., Steinsbø, Ø., Jahnsen, J., Lundin, K. E. A., Buus, S., Sollid, L. M., & Iversen, R. (2022). Focused B cell response to recurring gluten motif with implications for epitope spreading in celiac disease. Cell Reports, 41(4), [111541]. https://doi.org/10.1016/j.celrep.2022.111541

Vancouver

Zhou C, Østerbye T, Bach E, Dahal-Koirala S, Høydahl LS, Steinsbø Ø et al. Focused B cell response to recurring gluten motif with implications for epitope spreading in celiac disease. Cell Reports. 2022;41(4). 111541. https://doi.org/10.1016/j.celrep.2022.111541

Author

Zhou, Chunyan ; Østerbye, Thomas ; Bach, Emil ; Dahal-Koirala, Shiva ; Høydahl, Lene S. ; Steinsbø, Øyvind ; Jahnsen, Jørgen ; Lundin, Knut E.A. ; Buus, Søren ; Sollid, Ludvig M. ; Iversen, Rasmus. / Focused B cell response to recurring gluten motif with implications for epitope spreading in celiac disease. In: Cell Reports. 2022 ; Vol. 41, No. 4.

Bibtex

@article{90acc7b38e70429390583759be85c07f,

title = "Focused B cell response to recurring gluten motif with implications for epitope spreading in celiac disease",

abstract = "Antibodies to deamidated gluten peptides are accurate diagnostic markers of celiac disease. However, binding of patient antibodies to all possible gluten epitopes has not previously been investigated. Here, we assess serum antibody specificity across the gluten proteome by use of high-density peptide arrays. We confirm the importance of deamidation for antibody binding, and we show that the response is remarkably focused on the known epitope QPEQPFP (where E results from deamidation of Q). In addition, we describe an epitope in native (non-deamidated) gluten, QQPEQII (where E is gene encoded), which is associated with both B cell and T cell reactivity. Antibodies to this native epitope are cross-reactive with the major deamidated epitope due to recognition of the shared PEQ motif. Since cross-reactive B cells can present peptides to different gluten-specific T cells, we propose that such B cells play a role in epitope spreading by engaging T cells with multiple specificities.",

keywords = "antibodies, celiac disease, CP: Immunology, cross-reactivity, epitope spreading, gluten, peptide arrays, plasma cells, T cell-B cell collaboration",

author = "Chunyan Zhou and Thomas {\O}sterbye and Emil Bach and Shiva Dahal-Koirala and H{\o}ydahl, {Lene S.} and {\O}yvind Steinsb{\o} and J{\o}rgen Jahnsen and Lundin, {Knut E.A.} and S{\o}ren Buus and Sollid, {Ludvig M.} and Rasmus Iversen",

note = "Publisher Copyright: {\textcopyright} 2022 The Author(s)",

year = "2022",

doi = "10.1016/j.celrep.2022.111541",

language = "English",

volume = "41",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Cell Press",

number = "4",

}

RIS

TY - JOUR

T1 - Focused B cell response to recurring gluten motif with implications for epitope spreading in celiac disease

AU - Zhou, Chunyan

AU - Østerbye, Thomas

AU - Bach, Emil

AU - Dahal-Koirala, Shiva

AU - Høydahl, Lene S.

AU - Steinsbø, Øyvind

AU - Jahnsen, Jørgen

AU - Lundin, Knut E.A.

AU - Buus, Søren

AU - Sollid, Ludvig M.

AU - Iversen, Rasmus

PY - 2022

Y1 - 2022

N2 - Antibodies to deamidated gluten peptides are accurate diagnostic markers of celiac disease. However, binding of patient antibodies to all possible gluten epitopes has not previously been investigated. Here, we assess serum antibody specificity across the gluten proteome by use of high-density peptide arrays. We confirm the importance of deamidation for antibody binding, and we show that the response is remarkably focused on the known epitope QPEQPFP (where E results from deamidation of Q). In addition, we describe an epitope in native (non-deamidated) gluten, QQPEQII (where E is gene encoded), which is associated with both B cell and T cell reactivity. Antibodies to this native epitope are cross-reactive with the major deamidated epitope due to recognition of the shared PEQ motif. Since cross-reactive B cells can present peptides to different gluten-specific T cells, we propose that such B cells play a role in epitope spreading by engaging T cells with multiple specificities.

AB - Antibodies to deamidated gluten peptides are accurate diagnostic markers of celiac disease. However, binding of patient antibodies to all possible gluten epitopes has not previously been investigated. Here, we assess serum antibody specificity across the gluten proteome by use of high-density peptide arrays. We confirm the importance of deamidation for antibody binding, and we show that the response is remarkably focused on the known epitope QPEQPFP (where E results from deamidation of Q). In addition, we describe an epitope in native (non-deamidated) gluten, QQPEQII (where E is gene encoded), which is associated with both B cell and T cell reactivity. Antibodies to this native epitope are cross-reactive with the major deamidated epitope due to recognition of the shared PEQ motif. Since cross-reactive B cells can present peptides to different gluten-specific T cells, we propose that such B cells play a role in epitope spreading by engaging T cells with multiple specificities.

KW - antibodies

KW - celiac disease

KW - CP: Immunology

KW - cross-reactivity

KW - epitope spreading

KW - gluten

KW - peptide arrays

KW - plasma cells

KW - T cell-B cell collaboration

U2 - 10.1016/j.celrep.2022.111541

DO - 10.1016/j.celrep.2022.111541

M3 - Journal article

C2 - 36288703

AN - SCOPUS:85140401206

VL - 41

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 4

M1 - 111541

ER -

ID: 324317852

Forskning