TY - JOUR

T1 - Failure to demonstrate human T cell lymphotropic virus type I in multiple sclerosis patients

AU - Fugger, L

AU - Morling, N

AU - Ryder, L P

AU - Sandberg-Wollheim, M

AU - Svejgaard, A

N1 - Keywords: Base Sequence; Blotting, Southern; DNA, Viral; Female; Genes, env; Genes, gag; Genes, pol; Human T-lymphotropic virus 1; Humans; Male; Molecular Sequence Data; Multiple Sclerosis; Paraparesis, Tropical Spastic; Polymerase Chain Reaction

PY - 1990

Y1 - 1990

N2 - The polymerase chain reaction (PCR) technique was employed in searching for human T cell lymphotropic virus type I (HTLV-I) gag, env and pol sequences in samples of DNA prepared from two HTLV-I seropositive patients with tropical spastic paraparesis (TSP), the Swedish multiple sclerosis (MS) patients who recently have been reported to be PCR-positive for HTLV-I gag and env sequences, and eight healthy individuals. Precautions were taken in order to reduce the risk of cross-contamination in the PCR. In the two TSP patients strong signals were obtained with gag, env and pol amplification primers and detection probes. In MS patients and healthy individuals, no signals were obtained with gag and env. In occasional experiments, weak signals were seen for the pol segment for a single MS patient and/or healthy individuals, but these signals were not reproducible in subsequent experiments. Thus, the present data do not confirm the presence of HTLV-I sequences in MS patients.

AB - The polymerase chain reaction (PCR) technique was employed in searching for human T cell lymphotropic virus type I (HTLV-I) gag, env and pol sequences in samples of DNA prepared from two HTLV-I seropositive patients with tropical spastic paraparesis (TSP), the Swedish multiple sclerosis (MS) patients who recently have been reported to be PCR-positive for HTLV-I gag and env sequences, and eight healthy individuals. Precautions were taken in order to reduce the risk of cross-contamination in the PCR. In the two TSP patients strong signals were obtained with gag, env and pol amplification primers and detection probes. In MS patients and healthy individuals, no signals were obtained with gag and env. In occasional experiments, weak signals were seen for the pol segment for a single MS patient and/or healthy individuals, but these signals were not reproducible in subsequent experiments. Thus, the present data do not confirm the presence of HTLV-I sequences in MS patients.

M3 - Journal article

C2 - 2189023

VL - 71 ( Pt 5)

SP - 1103

EP - 1107

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

ER -