Expression, content, and localization of insulin-like growth factor I in human achilles tendon
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Expression, content, and localization of insulin-like growth factor I in human achilles tendon. / Olesen, Jens L; Heinemeier, Katja M; Langberg, Henning; Magnusson, S Peter; Kjaer, Michael; Flyvbjerg, Allan.
In: Connective Tissue Research, Vol. 47, No. 4, 2006, p. 200-206.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Expression, content, and localization of insulin-like growth factor I in human achilles tendon
AU - Olesen, Jens L
AU - Heinemeier, Katja M
AU - Langberg, Henning
AU - Magnusson, S Peter
AU - Kjaer, Michael
AU - Flyvbjerg, Allan
PY - 2006
Y1 - 2006
N2 - In animals insulin-like growth factor I (IGF-I) stimulates collagen production by fibroblasts and is expressed in tendons together with its binding protein 4 (IGFBP-4). However, the presence of IGF-I and IGFBP-4 in human tendon tissue is not described. Tissue IGF-I content was examined by immunoflourometric assay, real-time PCR, and immunohistochemistry used to localize and determine expression of IGF-I and IGFBP-4 in 6 postmortem human Achilles tendons. Tendon tissue concentrations of IGF-I were found to be 0.53 +/- 0.10 ng/g. Furthermore, we demonstrated that IGF-I and IGFBP-4 are localized around the tendon fibroblasts and that mRNA for IGF-I and IGFBP-4 can be determined in human tendon tissue. The present study adds support for the roles of IGF-I and IGFBP-4 in the regulation of tendon adaptive responses to mechanical loading.
AB - In animals insulin-like growth factor I (IGF-I) stimulates collagen production by fibroblasts and is expressed in tendons together with its binding protein 4 (IGFBP-4). However, the presence of IGF-I and IGFBP-4 in human tendon tissue is not described. Tissue IGF-I content was examined by immunoflourometric assay, real-time PCR, and immunohistochemistry used to localize and determine expression of IGF-I and IGFBP-4 in 6 postmortem human Achilles tendons. Tendon tissue concentrations of IGF-I were found to be 0.53 +/- 0.10 ng/g. Furthermore, we demonstrated that IGF-I and IGFBP-4 are localized around the tendon fibroblasts and that mRNA for IGF-I and IGFBP-4 can be determined in human tendon tissue. The present study adds support for the roles of IGF-I and IGFBP-4 in the regulation of tendon adaptive responses to mechanical loading.
KW - Achilles Tendon
KW - Adult
KW - Fluorescent Antibody Technique, Indirect
KW - Gene Expression
KW - Humans
KW - Insulin-Like Growth Factor Binding Protein 4
KW - Insulin-Like Growth Factor I
KW - Male
KW - RNA, Messenger
KW - Reverse Transcriptase Polymerase Chain Reaction
U2 - 10.1080/03008200600809893
DO - 10.1080/03008200600809893
M3 - Journal article
C2 - 16987751
VL - 47
SP - 200
EP - 206
JO - Connective Tissue Research
JF - Connective Tissue Research
SN - 0300-8207
IS - 4
ER -
ID: 38366164