Evaluation of an Enzyme Linked Immunosorbent Assay (ELISA) for Determination of Insulin in Dogs
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Evaluation of an Enzyme Linked Immunosorbent Assay (ELISA) for Determination of Insulin in Dogs. / HØIER, R.; JENSEN, A. L.
In: Journal of Veterinary Medicine Series A, Vol. 40, No. 1-10, 1993, p. 26-32.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Evaluation of an Enzyme Linked Immunosorbent Assay (ELISA) for Determination of Insulin in Dogs
AU - HØIER, R.
AU - JENSEN, A. L.
PY - 1993
Y1 - 1993
N2 - The purpose of the present study was to evaluate an enzyme linked immunosorbent assay (ELISA) technique for the determination of insulin in canine plasma samples. The test kit Enzymun‐Test®Boehringer Mannheim for determination of human insulin was used in connection with the analyzer system Enzymum‐Test®Boehringer Mannheim System ES‐33. The assay protocol suggested by the manufacturer was slightly modified by including an additional standard having a concentration within the canine reference range for insulin. Further, the incubation period for the immunoreaction and the enzymeassay was increased to 150 minutes and 90 minutes, respectively. When using the modified assay the concentration of insulin in canine plasma samples could be accurately and precisely determined with the exception, that the assay appeared to be somewhat imprecise (intraassay coefficient of variation being 22%) when lipaemic plasma samples were analysed. A slight overestimation of the actual concentrations (28%) was observed, but the measured concentrations of insulin in plasma samples from normal dogs compared favourable to those reported in literature. Eventually, a model for introduction of a species specific reference is discussed.
AB - The purpose of the present study was to evaluate an enzyme linked immunosorbent assay (ELISA) technique for the determination of insulin in canine plasma samples. The test kit Enzymun‐Test®Boehringer Mannheim for determination of human insulin was used in connection with the analyzer system Enzymum‐Test®Boehringer Mannheim System ES‐33. The assay protocol suggested by the manufacturer was slightly modified by including an additional standard having a concentration within the canine reference range for insulin. Further, the incubation period for the immunoreaction and the enzymeassay was increased to 150 minutes and 90 minutes, respectively. When using the modified assay the concentration of insulin in canine plasma samples could be accurately and precisely determined with the exception, that the assay appeared to be somewhat imprecise (intraassay coefficient of variation being 22%) when lipaemic plasma samples were analysed. A slight overestimation of the actual concentrations (28%) was observed, but the measured concentrations of insulin in plasma samples from normal dogs compared favourable to those reported in literature. Eventually, a model for introduction of a species specific reference is discussed.
UR - http://www.scopus.com/inward/record.url?scp=84995012940&partnerID=8YFLogxK
U2 - 10.1111/j.1439-0442.1993.tb00597.x
DO - 10.1111/j.1439-0442.1993.tb00597.x
M3 - Journal article
C2 - 8451901
AN - SCOPUS:84995012940
VL - 40
SP - 26
EP - 32
JO - Journal of Veterinary Medicine A
JF - Journal of Veterinary Medicine A
SN - 0931-184X
IS - 1-10
ER -
ID: 255559567