Elongator interactions with nascent mRNA revealed by RNA immunoprecipitation

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Standard

Elongator interactions with nascent mRNA revealed by RNA immunoprecipitation. / Gilbert, Christopher; Kristjuhan, Arnold; Winkler, G. Sebastiaan; Svejstrup, Jesper Q.

In: Molecular Cell, Vol. 14, No. 4, 21.05.2004, p. 457-464.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Gilbert, C, Kristjuhan, A, Winkler, GS & Svejstrup, JQ 2004, 'Elongator interactions with nascent mRNA revealed by RNA immunoprecipitation', Molecular Cell, vol. 14, no. 4, pp. 457-464. https://doi.org/10.1016/S1097-2765(04)00239-4

APA

Gilbert, C., Kristjuhan, A., Winkler, G. S., & Svejstrup, J. Q. (2004). Elongator interactions with nascent mRNA revealed by RNA immunoprecipitation. Molecular Cell, 14(4), 457-464. https://doi.org/10.1016/S1097-2765(04)00239-4

Vancouver

Gilbert C, Kristjuhan A, Winkler GS, Svejstrup JQ. Elongator interactions with nascent mRNA revealed by RNA immunoprecipitation. Molecular Cell. 2004 May 21;14(4):457-464. https://doi.org/10.1016/S1097-2765(04)00239-4

Author

Gilbert, Christopher ; Kristjuhan, Arnold ; Winkler, G. Sebastiaan ; Svejstrup, Jesper Q. / Elongator interactions with nascent mRNA revealed by RNA immunoprecipitation. In: Molecular Cell. 2004 ; Vol. 14, No. 4. pp. 457-464.

Bibtex

@article{96e8c435cce04a4aa7473aa469a53371,
title = "Elongator interactions with nascent mRNA revealed by RNA immunoprecipitation",
abstract = "The histone acetyltransferase Elongator was originally isolated as a component of the elongating form of RNA polymerase II (RNAPII) and a plethora of data has since supported a role for the factor in transcription. However, recent data has suggested that it is predominantly cytoplasmic and does not associate with the DNA of transcribed genes in vivo. Here, we report that Elongator binds to RNA both in vitro and in vivo. Using a modified chromatin immunoprecipitation procedure, RNA immunoprecipitation (RIP), we show that Elongator is indeed present at several actively transcribed genes and that it associates with the nascent RNA emanating from elongating RNAPII along the entire coding region of a gene. These results strongly support a role for Elongator in transcript elongation.",
author = "Christopher Gilbert and Arnold Kristjuhan and Winkler, {G. Sebastiaan} and Svejstrup, {Jesper Q.}",
note = "Funding Information: This project was supported by grants from Cancer Research UK and the European Union (HPRN-CT-2000-00087; to J.Q.S.) and by an EMBO Long-Term Fellowship (to G.S.W.). We thank Dirk G{\"o}rlich for a generous gift of anti-Cbp80 antibodies.",
year = "2004",
month = may,
day = "21",
doi = "10.1016/S1097-2765(04)00239-4",
language = "English",
volume = "14",
pages = "457--464",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "4",

}

RIS

TY - JOUR

T1 - Elongator interactions with nascent mRNA revealed by RNA immunoprecipitation

AU - Gilbert, Christopher

AU - Kristjuhan, Arnold

AU - Winkler, G. Sebastiaan

AU - Svejstrup, Jesper Q.

N1 - Funding Information: This project was supported by grants from Cancer Research UK and the European Union (HPRN-CT-2000-00087; to J.Q.S.) and by an EMBO Long-Term Fellowship (to G.S.W.). We thank Dirk Görlich for a generous gift of anti-Cbp80 antibodies.

PY - 2004/5/21

Y1 - 2004/5/21

N2 - The histone acetyltransferase Elongator was originally isolated as a component of the elongating form of RNA polymerase II (RNAPII) and a plethora of data has since supported a role for the factor in transcription. However, recent data has suggested that it is predominantly cytoplasmic and does not associate with the DNA of transcribed genes in vivo. Here, we report that Elongator binds to RNA both in vitro and in vivo. Using a modified chromatin immunoprecipitation procedure, RNA immunoprecipitation (RIP), we show that Elongator is indeed present at several actively transcribed genes and that it associates with the nascent RNA emanating from elongating RNAPII along the entire coding region of a gene. These results strongly support a role for Elongator in transcript elongation.

AB - The histone acetyltransferase Elongator was originally isolated as a component of the elongating form of RNA polymerase II (RNAPII) and a plethora of data has since supported a role for the factor in transcription. However, recent data has suggested that it is predominantly cytoplasmic and does not associate with the DNA of transcribed genes in vivo. Here, we report that Elongator binds to RNA both in vitro and in vivo. Using a modified chromatin immunoprecipitation procedure, RNA immunoprecipitation (RIP), we show that Elongator is indeed present at several actively transcribed genes and that it associates with the nascent RNA emanating from elongating RNAPII along the entire coding region of a gene. These results strongly support a role for Elongator in transcript elongation.

U2 - 10.1016/S1097-2765(04)00239-4

DO - 10.1016/S1097-2765(04)00239-4

M3 - Journal article

C2 - 15149595

AN - SCOPUS:2442425954

VL - 14

SP - 457

EP - 464

JO - Molecular Cell

JF - Molecular Cell

SN - 1097-2765

IS - 4

ER -

ID: 331040832