Effects of G-CSF on telomere lengths in PBMCs from human immunodeficiency virus-infected patients: results from a randomized, placebo-controlled trial
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Effects of G-CSF on telomere lengths in PBMCs from human immunodeficiency virus-infected patients : results from a randomized, placebo-controlled trial. / Aladdin, H; Ullum, H; Schjerling, P; Skov Jensen, M; Dam Nielsen, S.; Mathiesen, L; Gerstoft, J; Skinhøj, P; Klarlund Pedersen, B.
In: Scandinavian Journal of Immunology, Vol. 52, No. 2, 08.2000, p. 212-6.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Effects of G-CSF on telomere lengths in PBMCs from human immunodeficiency virus-infected patients
T2 - results from a randomized, placebo-controlled trial
AU - Aladdin, H
AU - Ullum, H
AU - Schjerling, P
AU - Skov Jensen, M
AU - Dam Nielsen, S.
AU - Mathiesen, L
AU - Gerstoft, J
AU - Skinhøj, P
AU - Klarlund Pedersen, B
PY - 2000/8
Y1 - 2000/8
N2 - Telomeres are unique terminal chromosomal structures, the length of which has been shown to decrease with cell division in vitro and with increased age in vivo for human somatic cells. In human immunodeficiency virus (HIV)-1 infection, decrease of telomere length is primarily found in CD8+ T cells, and not in CD4+ T cells. In this double-blind placebo-controlled study, we investigated the effect of granulocyte colony stimulating factor (G-CSF) treatment combined with highly active antiretroviral therapy (HAART) on mean telomere length in peripheral blood mononuclear cells (PBMC). The terminal restriction fragment (TRF) length showed no changes during G-CSF treatment although the number of lymphocytes increased significantly. The mean TRF length correlated positively (R = 0.552, P = 0.009) and negatively (R = -0.503, P = 0.02) to the proportion of CD4+ memory and naïve cells, respectively. Our data suggest that during G-CSF treatment lymphocytes are recruited by a combination of central and peripheral proliferation.
AB - Telomeres are unique terminal chromosomal structures, the length of which has been shown to decrease with cell division in vitro and with increased age in vivo for human somatic cells. In human immunodeficiency virus (HIV)-1 infection, decrease of telomere length is primarily found in CD8+ T cells, and not in CD4+ T cells. In this double-blind placebo-controlled study, we investigated the effect of granulocyte colony stimulating factor (G-CSF) treatment combined with highly active antiretroviral therapy (HAART) on mean telomere length in peripheral blood mononuclear cells (PBMC). The terminal restriction fragment (TRF) length showed no changes during G-CSF treatment although the number of lymphocytes increased significantly. The mean TRF length correlated positively (R = 0.552, P = 0.009) and negatively (R = -0.503, P = 0.02) to the proportion of CD4+ memory and naïve cells, respectively. Our data suggest that during G-CSF treatment lymphocytes are recruited by a combination of central and peripheral proliferation.
KW - Adult
KW - Aged
KW - Anti-HIV Agents
KW - CD4 Lymphocyte Count
KW - Double-Blind Method
KW - Drug Therapy, Combination
KW - Female
KW - Granulocyte Colony-Stimulating Factor
KW - HIV Infections
KW - Humans
KW - In Vitro Techniques
KW - Leukocytes, Mononuclear
KW - Lymphocytes
KW - Male
KW - Middle Aged
KW - Monocytes
KW - Telomere
KW - Clinical Trial
KW - Journal Article
KW - Randomized Controlled Trial
KW - Research Support, Non-U.S. Gov't
M3 - Journal article
C2 - 10931390
VL - 52
SP - 212
EP - 216
JO - Scandinavian Journal of Immunology, Supplement
JF - Scandinavian Journal of Immunology, Supplement
SN - 0301-6323
IS - 2
ER -
ID: 180571884