Effect of Roux-en-Y gastric bypass and laparoscopic adjustable gastric banding on branched-chain amino acid metabolism
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Effect of Roux-en-Y gastric bypass and laparoscopic adjustable gastric banding on branched-chain amino acid metabolism. / Magkos, Faidon; Bradley, David; Schweitzer, George G; Finck, Brian N; Eagon, J Christopher; Ilkayeva, Olga; Newgard, Christopher B; Klein, Samuel.
In: Diabetes, Vol. 62, No. 8, 2013, p. 2757-2761.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Effect of Roux-en-Y gastric bypass and laparoscopic adjustable gastric banding on branched-chain amino acid metabolism
AU - Magkos, Faidon
AU - Bradley, David
AU - Schweitzer, George G
AU - Finck, Brian N
AU - Eagon, J Christopher
AU - Ilkayeva, Olga
AU - Newgard, Christopher B
AU - Klein, Samuel
N1 - (Ekstern)
PY - 2013
Y1 - 2013
N2 - It has been hypothesized that a greater decline in circulating branched-chain amino acids (BCAAs) after weight loss induced by Roux-en-Y gastric bypass (RYGB) surgery than after calorie restriction alone has independent effects on glucose homeostasis, possibly by decreased signaling through the mammalian target of rapamycin (mTOR). We evaluated plasma BCAAs and their C3 and C5 acylcarnitine metabolites, muscle mTOR phosphorylation, and insulin sensitivity (insulin-stimulated glucose Rd) in obese subjects before and after ~20% weight loss induced by RYGB (n = 10, BMI 45.6 ± 6.7 kg/m(2)) or laparoscopic adjustable gastric banding (LAGB) (n = 10, BMI 46.5 ± 8.8 kg/m(2)). Weight loss increased insulin-stimulated glucose Rd by ~55%, decreased total plasma BCAA and C3 and C5 acylcarnitine concentrations by 20-35%, and did not alter mTOR phosphorylation; no differences were detected between surgical groups (all P values for interaction >0.05). Insulin-stimulated glucose Rd correlated negatively with plasma BCAAs and with C3 and C5 acylcarnitine concentrations (r values -0.56 to -0.75, P < 0.05). These data demonstrate that weight loss induced by either LAGB or RYGB causes the same decline in circulating BCAAs and their C3 and C5 acylcarnitine metabolites. Plasma BCAA concentration is negatively associated with skeletal muscle insulin sensitivity, but the mechanism(s) responsible for this relationship is not known.
AB - It has been hypothesized that a greater decline in circulating branched-chain amino acids (BCAAs) after weight loss induced by Roux-en-Y gastric bypass (RYGB) surgery than after calorie restriction alone has independent effects on glucose homeostasis, possibly by decreased signaling through the mammalian target of rapamycin (mTOR). We evaluated plasma BCAAs and their C3 and C5 acylcarnitine metabolites, muscle mTOR phosphorylation, and insulin sensitivity (insulin-stimulated glucose Rd) in obese subjects before and after ~20% weight loss induced by RYGB (n = 10, BMI 45.6 ± 6.7 kg/m(2)) or laparoscopic adjustable gastric banding (LAGB) (n = 10, BMI 46.5 ± 8.8 kg/m(2)). Weight loss increased insulin-stimulated glucose Rd by ~55%, decreased total plasma BCAA and C3 and C5 acylcarnitine concentrations by 20-35%, and did not alter mTOR phosphorylation; no differences were detected between surgical groups (all P values for interaction >0.05). Insulin-stimulated glucose Rd correlated negatively with plasma BCAAs and with C3 and C5 acylcarnitine concentrations (r values -0.56 to -0.75, P < 0.05). These data demonstrate that weight loss induced by either LAGB or RYGB causes the same decline in circulating BCAAs and their C3 and C5 acylcarnitine metabolites. Plasma BCAA concentration is negatively associated with skeletal muscle insulin sensitivity, but the mechanism(s) responsible for this relationship is not known.
KW - Adult
KW - Amino Acids, Branched-Chain/metabolism
KW - Blood Glucose/metabolism
KW - Caloric Restriction
KW - Female
KW - Gastric Bypass
KW - Gastroplasty
KW - Humans
KW - Insulin/pharmacology
KW - Insulin Resistance
KW - Laparoscopy
KW - Male
KW - Muscle, Skeletal/metabolism
KW - Obesity/metabolism
KW - Phosphorylation
KW - TOR Serine-Threonine Kinases/metabolism
U2 - 10.2337/db13-0185
DO - 10.2337/db13-0185
M3 - Journal article
C2 - 23610059
VL - 62
SP - 2757
EP - 2761
JO - Diabetes
JF - Diabetes
SN - 0012-1797
IS - 8
ER -
ID: 289967843