TY - JOUR

T1 - Drug distribution in spinal cord during administration with spinal loop dialysis probes in anaesthetized rats

AU - Uustalu, Maria

AU - Abelson, Klas S P

PY - 2007/3

Y1 - 2007/3

N2 - The present investigation aimed to study two methodological concerns of an experimental model, where a spinal loop dialysis probe is used for administration of substances to the spinal cord and sampling of neurotransmitters by microdialysis from the same area of anaesthetized rats. [(3)H]Epibatidine in concentrations of 1, 10 and 100 nM was dissolved in Ringer's solution and administered through the dialysis membrane into the dorsal region of the cervical spinal cord. First, the outflow of [(3)H]epibatidine from the probe into the spinal cord was examined with respect to different concentrations and changes over time. Then, the distribution of the different [(3)H]epibatidine concentrations along the spinal cord was studied. It was found that the percentage of [(3)H]epibatidine entering the spinal cord did not differ between different administered concentrations after a stabilization period of 60 min. The administered [(3)H]epibatidine was found to be distributed to the area closest to the dialysis probe and not dispersed along the spinal cord, and the distribution was equal for all concentrations. The data presented in this investigation provide information, which is important for interpretation of data from intraspinal administration of substances through the spinal loop dialysis probe.

AB - The present investigation aimed to study two methodological concerns of an experimental model, where a spinal loop dialysis probe is used for administration of substances to the spinal cord and sampling of neurotransmitters by microdialysis from the same area of anaesthetized rats. [(3)H]Epibatidine in concentrations of 1, 10 and 100 nM was dissolved in Ringer's solution and administered through the dialysis membrane into the dorsal region of the cervical spinal cord. First, the outflow of [(3)H]epibatidine from the probe into the spinal cord was examined with respect to different concentrations and changes over time. Then, the distribution of the different [(3)H]epibatidine concentrations along the spinal cord was studied. It was found that the percentage of [(3)H]epibatidine entering the spinal cord did not differ between different administered concentrations after a stabilization period of 60 min. The administered [(3)H]epibatidine was found to be distributed to the area closest to the dialysis probe and not dispersed along the spinal cord, and the distribution was equal for all concentrations. The data presented in this investigation provide information, which is important for interpretation of data from intraspinal administration of substances through the spinal loop dialysis probe.

KW - Analgesics, Non-Narcotic

KW - Animals

KW - Bicyclo Compounds, Heterocyclic

KW - Dose-Response Relationship, Drug

KW - Injections, Spinal

KW - Isotonic Solutions

KW - Male

KW - Microdialysis

KW - Models, Animal

KW - Pyridines

KW - Rats

KW - Rats, Sprague-Dawley

KW - Spinal Cord

KW - Tissue Distribution

U2 - 10.1111/j.1742-7843.2006.00034.x

DO - 10.1111/j.1742-7843.2006.00034.x

M3 - Journal article

C2 - 17309524

VL - 100

SP - 196

EP - 200

JO - Basic and Clinical Pharmacology and Toxicology

JF - Basic and Clinical Pharmacology and Toxicology

SN - 1742-7835

IS - 3

ER -