Dose dependent gene expression is dynamically modulated by the history, physiology and age of yeast cells
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Dose dependent gene expression is dynamically modulated by the history, physiology and age of yeast cells. / Pascual-Ahuir, Amparo; González-Cantó, Eva; Juyoux, Pauline; Pable, Julia; Poveda-Huertes, Daniel; Saiz-Balbastre, Sandra; Squeo, Sonia; Ureña-Marco, Alvaro; Vanacloig-Pedros, Elena; Zaragoza-Infante, Laura; Proft, Markus.
In: BBA Gene Regulatory Mechanisms, Vol. 1862, No. 4, 2019, p. 457-471.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Dose dependent gene expression is dynamically modulated by the history, physiology and age of yeast cells
AU - Pascual-Ahuir, Amparo
AU - González-Cantó, Eva
AU - Juyoux, Pauline
AU - Pable, Julia
AU - Poveda-Huertes, Daniel
AU - Saiz-Balbastre, Sandra
AU - Squeo, Sonia
AU - Ureña-Marco, Alvaro
AU - Vanacloig-Pedros, Elena
AU - Zaragoza-Infante, Laura
AU - Proft, Markus
N1 - Copyright © 2019 Elsevier B.V. All rights reserved.
PY - 2019
Y1 - 2019
N2 - Cells respond to external stimuli with transient gene expression changes in order to adapt to environmental alterations. However, the dose response profile of gene induction upon a given stress depends on many intrinsic and extrinsic factors. Here we show that the accurate quantification of dose dependent gene expression by live cell luciferase reporters reveals fundamental insights into stress signaling. We make the following discoveries applying this non-invasive reporter technology. (1) Signal transduction sensitivities can be compared and we apply this here to salt, oxidative and xenobiotic stress responsive transcription factors. (2) Stress signaling depends on where and how the damage is generated within the cell. Specifically we show that two ROS-generating agents, menadione and hydrogen peroxide, differ in their dependence on mitochondrial respiration. (3) Stress signaling is conditioned by the cells history. We demonstrate here that positive memory or an acquired resistance towards oxidative stress is induced dependent on the nature of the previous stress experience. (4) The metabolic state of the cell impinges on the sensitivity of stress signaling. This is shown here for the shift towards higher stress doses of the response profile for yeast cells moved from complex to synthetic medium. (5) The age of the cell conditions its transcriptional response capacity, which is demonstrated by the changes of the dose response to oxidative stress during both replicative and chronological aging. We conclude that capturing dose dependent gene expression in real time will be of invaluable help to understand stress signaling and its dynamic modulation.
AB - Cells respond to external stimuli with transient gene expression changes in order to adapt to environmental alterations. However, the dose response profile of gene induction upon a given stress depends on many intrinsic and extrinsic factors. Here we show that the accurate quantification of dose dependent gene expression by live cell luciferase reporters reveals fundamental insights into stress signaling. We make the following discoveries applying this non-invasive reporter technology. (1) Signal transduction sensitivities can be compared and we apply this here to salt, oxidative and xenobiotic stress responsive transcription factors. (2) Stress signaling depends on where and how the damage is generated within the cell. Specifically we show that two ROS-generating agents, menadione and hydrogen peroxide, differ in their dependence on mitochondrial respiration. (3) Stress signaling is conditioned by the cells history. We demonstrate here that positive memory or an acquired resistance towards oxidative stress is induced dependent on the nature of the previous stress experience. (4) The metabolic state of the cell impinges on the sensitivity of stress signaling. This is shown here for the shift towards higher stress doses of the response profile for yeast cells moved from complex to synthetic medium. (5) The age of the cell conditions its transcriptional response capacity, which is demonstrated by the changes of the dose response to oxidative stress during both replicative and chronological aging. We conclude that capturing dose dependent gene expression in real time will be of invaluable help to understand stress signaling and its dynamic modulation.
KW - Gene Expression Regulation, Fungal
KW - Genes, Reporter
KW - Luciferases/genetics
KW - Osmotic Pressure
KW - Oxidative Stress/genetics
KW - Saccharomyces cerevisiae/genetics
KW - Signal Transduction
KW - Transcription Factors/metabolism
KW - Transcription, Genetic
U2 - 10.1016/j.bbagrm.2019.02.009
DO - 10.1016/j.bbagrm.2019.02.009
M3 - Journal article
C2 - 30836134
VL - 1862
SP - 457
EP - 471
JO - BBA Gene Regulatory Mechanisms
JF - BBA Gene Regulatory Mechanisms
SN - 1874-9399
IS - 4
ER -
ID: 391635048