DNA conformational analysis in solution by uranyl mediated photocleavage
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DNA conformational analysis in solution by uranyl mediated photocleavage. / Nielsen, Peter E.; Møllegaard, N E; Jeppesen, C.
In: Nucleic Acids Research, Vol. 18, No. 13, 1990, p. 3847-51.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - DNA conformational analysis in solution by uranyl mediated photocleavage
AU - Nielsen, Peter E.
AU - Møllegaard, N E
AU - Jeppesen, C
N1 - Keywords: Animals; Base Sequence; DNA; Densitometry; Molecular Sequence Data; Nucleic Acid Conformation; Photochemistry; Uranium; Uranyl Nitrate; Xenopus
PY - 1990
Y1 - 1990
N2 - Uranyl mediated photocleavage of double stranded DNA is proposed as a general probing for DNA helix conformation in terms of minor groove width/electronegative potential. Specifically, it is found that A/T-tracts known to constitute strong distamycin binding sites are preferentially photocleaved by uranyl in a way indicating strongest uranyl binding at the center of the minor groove of the AT-region. The A-tracts of kinetoplast DNA show the highest reactivity at the 3'-end of the tract--as opposed to cleavage by EDTA/Fell--in accordance with the minor groove being more narrow at this end. Finally, uranyl photocleavage of the internal control region (ICR) of the 5S-RNA gene yields a cleavage modulation pattern fully compatible with that obtained by DNase I which also--in a more complex way--senses DNA minor groove width.
AB - Uranyl mediated photocleavage of double stranded DNA is proposed as a general probing for DNA helix conformation in terms of minor groove width/electronegative potential. Specifically, it is found that A/T-tracts known to constitute strong distamycin binding sites are preferentially photocleaved by uranyl in a way indicating strongest uranyl binding at the center of the minor groove of the AT-region. The A-tracts of kinetoplast DNA show the highest reactivity at the 3'-end of the tract--as opposed to cleavage by EDTA/Fell--in accordance with the minor groove being more narrow at this end. Finally, uranyl photocleavage of the internal control region (ICR) of the 5S-RNA gene yields a cleavage modulation pattern fully compatible with that obtained by DNase I which also--in a more complex way--senses DNA minor groove width.
M3 - Journal article
C2 - 2374711
VL - 18
SP - 3847
EP - 3851
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 13
ER -
ID: 9828913