Diurnal variation of urinary markers of nucleic acid oxidation
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Diurnal variation of urinary markers of nucleic acid oxidation. / Grew, Ida S; Cejvanovic, Vanja; Broedbaek, Kasper; Henriksen, Trine; Petersen, Morten; Andersen, Jon T; Jimenez-Solem, Espen; Weimann, Allan; Poulsen, Henrik E.
In: Scandinavian Journal of Clinical & Laboratory Investigation, Vol. 74, No. 4, 06.2014, p. 336-343.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Diurnal variation of urinary markers of nucleic acid oxidation
AU - Grew, Ida S
AU - Cejvanovic, Vanja
AU - Broedbaek, Kasper
AU - Henriksen, Trine
AU - Petersen, Morten
AU - Andersen, Jon T
AU - Jimenez-Solem, Espen
AU - Weimann, Allan
AU - Poulsen, Henrik E
PY - 2014/6
Y1 - 2014/6
N2 - AIMS: Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydroguanosine (8-oxoGuo) are biomarkers of oxidative stress with clinical potential in a variety of diseases. As part of their clinical validation, this study aimed to investigate whether the urinary excretion of 8-oxodG and 8-oxoGuo undergoes diurnal variation and to evaluate the validity of 6-hour sampling as well as creatinine corrected spot urine sampling.METHODS: A total of 23 healthy study subjects collecting their 24-h urine in four fractions covering 6 hours each. Urinary 8-oxodG and 8-oxoGuo levels were quantified using a modified version of UPLC-MS/MS.RESULTS: No significant difference in excretion levels between the 12-h diurnal and 12-h nocturnal state or between the four 6-h periods during the day was found for either biomarker. A strong linear relationship between the excretion levels in each of the 6-h periods and the 24-h excretion level was shown for both biomarkers. Creatinine correction of the 6-h levels reduced the biological variation of the excretion levels and weakened the linear relationship with the uncorrected 24-h excretion level for both biomarkers. The correlations were strengthened when the 24-h excretion level was expressed per kg body weight.CONCLUSION: The results showed that 8-oxodG and 8-oxoGuo did not undergo diurnal variation in the study population overall and hence that the time of sampling is not crucial. Furthermore, 6-h sampling can be used as a substitute for 24-h sampling, and creatinine corrected sampling may be rational due to the reduction in biological variation of the biomarkers and the reasonable correlation with body weight-adjusted 24-h levels.
AB - AIMS: Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydroguanosine (8-oxoGuo) are biomarkers of oxidative stress with clinical potential in a variety of diseases. As part of their clinical validation, this study aimed to investigate whether the urinary excretion of 8-oxodG and 8-oxoGuo undergoes diurnal variation and to evaluate the validity of 6-hour sampling as well as creatinine corrected spot urine sampling.METHODS: A total of 23 healthy study subjects collecting their 24-h urine in four fractions covering 6 hours each. Urinary 8-oxodG and 8-oxoGuo levels were quantified using a modified version of UPLC-MS/MS.RESULTS: No significant difference in excretion levels between the 12-h diurnal and 12-h nocturnal state or between the four 6-h periods during the day was found for either biomarker. A strong linear relationship between the excretion levels in each of the 6-h periods and the 24-h excretion level was shown for both biomarkers. Creatinine correction of the 6-h levels reduced the biological variation of the excretion levels and weakened the linear relationship with the uncorrected 24-h excretion level for both biomarkers. The correlations were strengthened when the 24-h excretion level was expressed per kg body weight.CONCLUSION: The results showed that 8-oxodG and 8-oxoGuo did not undergo diurnal variation in the study population overall and hence that the time of sampling is not crucial. Furthermore, 6-h sampling can be used as a substitute for 24-h sampling, and creatinine corrected sampling may be rational due to the reduction in biological variation of the biomarkers and the reasonable correlation with body weight-adjusted 24-h levels.
KW - Adult
KW - Aged
KW - Biological Markers
KW - Circadian Rhythm
KW - Creatinine
KW - Deoxyguanosine
KW - Female
KW - Guanosine
KW - Humans
KW - Male
KW - Middle Aged
KW - Oxidation-Reduction
KW - Oxidative Stress
KW - Reproducibility of Results
U2 - 10.3109/00365513.2014.891258
DO - 10.3109/00365513.2014.891258
M3 - Journal article
C2 - 24628455
VL - 74
SP - 336
EP - 343
JO - Scandinavian Journal of Clinical & Laboratory Investigation
JF - Scandinavian Journal of Clinical & Laboratory Investigation
SN - 0036-5513
IS - 4
ER -
ID: 137429087