TY - JOUR

T1 - Distribution of the UV filter 3-benzylidene camphor in rat following topical application

AU - Søeborg, Tue

AU - Ganderup, Niels Christian

AU - Kristensen, Jakob Højer

AU - Bjerregaard, Poul

AU - Pedersen, Knud Ladegaard

AU - Bollen, Peter

AU - Hansen, Steen Honoré

AU - Halling-Sørensen, Bent

PY - 2006

Y1 - 2006

N2 - A straightforward analytical method for determination of 3-benzylidene camphor (3-BC) in rat adipose tissue, brain, liver, muscle, plasma and testis following topical application was developed and validated. Three exposure levels (60, 180 and 540 mg kg-1 day-1) were tested for 65 days in male Sprague-Dawley rats (24 days postnatal). Sample preparation involving homogenization and n-heptane or methanol extraction of the tissue was applied before injection into the LC-ESI-MS-MS system. The response was linear from 2 to 100 μg l-1 for the qualifier and the quantifier MRM transitions (R2 (quantifier) > 0.994). Detection limit of the method corresponded to 0.005 μg g-1 tissue and 12.5 μg l-1 plasma, respectively. Recovery was determined for all tissues (adipose tissue: 40%; all other tissues: 80-100%) at three individual levels. 3-(4-Methyl benzylidene camphor) (4-MBC) was used throughout the study as internal standard. 3-Benzylidene camphor was detected in all tissues at all exposure levels at concentrations between 0.05 μg g-1 (liver) and 36 μg g-1 (adipose tissue) and in plasma at 16-89 μg l-1. The method allowed for the quantification of 3-benzylidene camphor in all tested tissues following topical application. Furthermore, it was shown that 3-benzylidene camphor can be found in various tissues in the rat following topical application. These findings may suggest that following use of 3-benzylidene camphor containing sunscreen, similar disposition and distribution may occur in humans.

AB - A straightforward analytical method for determination of 3-benzylidene camphor (3-BC) in rat adipose tissue, brain, liver, muscle, plasma and testis following topical application was developed and validated. Three exposure levels (60, 180 and 540 mg kg-1 day-1) were tested for 65 days in male Sprague-Dawley rats (24 days postnatal). Sample preparation involving homogenization and n-heptane or methanol extraction of the tissue was applied before injection into the LC-ESI-MS-MS system. The response was linear from 2 to 100 μg l-1 for the qualifier and the quantifier MRM transitions (R2 (quantifier) > 0.994). Detection limit of the method corresponded to 0.005 μg g-1 tissue and 12.5 μg l-1 plasma, respectively. Recovery was determined for all tissues (adipose tissue: 40%; all other tissues: 80-100%) at three individual levels. 3-(4-Methyl benzylidene camphor) (4-MBC) was used throughout the study as internal standard. 3-Benzylidene camphor was detected in all tissues at all exposure levels at concentrations between 0.05 μg g-1 (liver) and 36 μg g-1 (adipose tissue) and in plasma at 16-89 μg l-1. The method allowed for the quantification of 3-benzylidene camphor in all tested tissues following topical application. Furthermore, it was shown that 3-benzylidene camphor can be found in various tissues in the rat following topical application. These findings may suggest that following use of 3-benzylidene camphor containing sunscreen, similar disposition and distribution may occur in humans.

KW - Adipose tissue

KW - Brain

KW - Estrogenic effect

KW - In vivo

KW - Liver

KW - Mass spectrometry

KW - Muscle

KW - Plasma

KW - Sunscreen

KW - Testis

U2 - 10.1016/j.jchromb.2006.02.026

DO - 10.1016/j.jchromb.2006.02.026

M3 - Journal article

C2 - 16517225

AN - SCOPUS:33646002684

VL - 834

SP - 117

EP - 121

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

IS - 1-2

ER -