Distribution of cytochrome P450 2C, 2E1, 3A4, and 3A5 in human colon mucosa

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Distribution of cytochrome P450 2C, 2E1, 3A4, and 3A5 in human colon mucosa. / Bergheim, Ina; Bode, Christiane; Parlesak, Alexandr.

In: BMC Clinical Pharmacology, Vol. 5, 4, 2005.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bergheim, I, Bode, C & Parlesak, A 2005, 'Distribution of cytochrome P450 2C, 2E1, 3A4, and 3A5 in human colon mucosa', BMC Clinical Pharmacology, vol. 5, 4. https://doi.org/10.1186/1472-6904-5-4

APA

Bergheim, I., Bode, C., & Parlesak, A. (2005). Distribution of cytochrome P450 2C, 2E1, 3A4, and 3A5 in human colon mucosa. BMC Clinical Pharmacology, 5, [4]. https://doi.org/10.1186/1472-6904-5-4

Vancouver

Bergheim I, Bode C, Parlesak A. Distribution of cytochrome P450 2C, 2E1, 3A4, and 3A5 in human colon mucosa. BMC Clinical Pharmacology. 2005;5. 4. https://doi.org/10.1186/1472-6904-5-4

Author

Bergheim, Ina ; Bode, Christiane ; Parlesak, Alexandr. / Distribution of cytochrome P450 2C, 2E1, 3A4, and 3A5 in human colon mucosa. In: BMC Clinical Pharmacology. 2005 ; Vol. 5.

Bibtex

@article{f81bece4402844e0b900b381b54a829b,
title = "Distribution of cytochrome P450 2C, 2E1, 3A4, and 3A5 in human colon mucosa",
abstract = "Background: Despite the fact that the alimentary tract is part of the body's first line of defense against orally ingested xenobiotica, little is known about the distribution and expression of cytochrome P450 (CYP) enzymes in human colon. Therefore, expression and protein levels of four representative CYPs (CYP2C(8), CYP2E1, CYP3A4, and CYP3A5) were determined in human colon mucosa biopsies obtained from ascending, descending and sigmoid colon.Methods: Expression of CYP2C, CYP2E1, CYP3A4, and CYP3A5 mRNA in colon mucosa was determined by RT-PCR. Protein concentration of CYPs was determined using Western blot methods.Results: Extensive interindividual variability was found for the expression of most of the genes. However, expression of CYP2C mRNA levels were significantly higher in the ascending colon than in the sigmoid colon. In contrast, mRNA levels of CYP2E1 and CYP3A5 were significantly lower in the ascending colon in comparison to the descending and sigmoid colon. In sigmoid colon proteinlevels of CYP2C8 were significantly higher by ~73% than in the descending colon. In contrast, protein concentration of CYP2E1 was significantly lower by ~81% in the sigmoid colon in comparison to the descending colon.Conclusion: The current data suggest that the expression of CYP2C, CYP2E1, and CYP3A5 varies in different parts of the colon.",
author = "Ina Bergheim and Christiane Bode and Alexandr Parlesak",
note = "(Ekstern)",
year = "2005",
doi = "10.1186/1472-6904-5-4",
language = "English",
volume = "5",
journal = "BMC pharmacology & toxicology",
issn = "2050-6511",
publisher = "BioMed Central",

}

RIS

TY - JOUR

T1 - Distribution of cytochrome P450 2C, 2E1, 3A4, and 3A5 in human colon mucosa

AU - Bergheim, Ina

AU - Bode, Christiane

AU - Parlesak, Alexandr

N1 - (Ekstern)

PY - 2005

Y1 - 2005

N2 - Background: Despite the fact that the alimentary tract is part of the body's first line of defense against orally ingested xenobiotica, little is known about the distribution and expression of cytochrome P450 (CYP) enzymes in human colon. Therefore, expression and protein levels of four representative CYPs (CYP2C(8), CYP2E1, CYP3A4, and CYP3A5) were determined in human colon mucosa biopsies obtained from ascending, descending and sigmoid colon.Methods: Expression of CYP2C, CYP2E1, CYP3A4, and CYP3A5 mRNA in colon mucosa was determined by RT-PCR. Protein concentration of CYPs was determined using Western blot methods.Results: Extensive interindividual variability was found for the expression of most of the genes. However, expression of CYP2C mRNA levels were significantly higher in the ascending colon than in the sigmoid colon. In contrast, mRNA levels of CYP2E1 and CYP3A5 were significantly lower in the ascending colon in comparison to the descending and sigmoid colon. In sigmoid colon proteinlevels of CYP2C8 were significantly higher by ~73% than in the descending colon. In contrast, protein concentration of CYP2E1 was significantly lower by ~81% in the sigmoid colon in comparison to the descending colon.Conclusion: The current data suggest that the expression of CYP2C, CYP2E1, and CYP3A5 varies in different parts of the colon.

AB - Background: Despite the fact that the alimentary tract is part of the body's first line of defense against orally ingested xenobiotica, little is known about the distribution and expression of cytochrome P450 (CYP) enzymes in human colon. Therefore, expression and protein levels of four representative CYPs (CYP2C(8), CYP2E1, CYP3A4, and CYP3A5) were determined in human colon mucosa biopsies obtained from ascending, descending and sigmoid colon.Methods: Expression of CYP2C, CYP2E1, CYP3A4, and CYP3A5 mRNA in colon mucosa was determined by RT-PCR. Protein concentration of CYPs was determined using Western blot methods.Results: Extensive interindividual variability was found for the expression of most of the genes. However, expression of CYP2C mRNA levels were significantly higher in the ascending colon than in the sigmoid colon. In contrast, mRNA levels of CYP2E1 and CYP3A5 were significantly lower in the ascending colon in comparison to the descending and sigmoid colon. In sigmoid colon proteinlevels of CYP2C8 were significantly higher by ~73% than in the descending colon. In contrast, protein concentration of CYP2E1 was significantly lower by ~81% in the sigmoid colon in comparison to the descending colon.Conclusion: The current data suggest that the expression of CYP2C, CYP2E1, and CYP3A5 varies in different parts of the colon.

U2 - 10.1186/1472-6904-5-4

DO - 10.1186/1472-6904-5-4

M3 - Journal article

VL - 5

JO - BMC pharmacology & toxicology

JF - BMC pharmacology & toxicology

SN - 2050-6511

M1 - 4

ER -

ID: 317460249