Different Mechanisms Confer Gradual Control and Memory at Nutrient- and Stress-Regulated Genes in Yeast
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Different Mechanisms Confer Gradual Control and Memory at Nutrient- and Stress-Regulated Genes in Yeast. / Rienzo, Alessandro; Poveda-Huertes, Daniel; Aydin, Selcan; Buchler, Nicolas E; Pascual-Ahuir, Amparo; Proft, Markus.
In: Molecular and Cellular Biology, Vol. 35, No. 21, 11.2015, p. 3669-83.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Different Mechanisms Confer Gradual Control and Memory at Nutrient- and Stress-Regulated Genes in Yeast
AU - Rienzo, Alessandro
AU - Poveda-Huertes, Daniel
AU - Aydin, Selcan
AU - Buchler, Nicolas E
AU - Pascual-Ahuir, Amparo
AU - Proft, Markus
N1 - Copyright © 2015, American Society for Microbiology. All Rights Reserved.
PY - 2015/11
Y1 - 2015/11
N2 - Cells respond to environmental stimuli by fine-tuned regulation of gene expression. Here we investigated the dose-dependent modulation of gene expression at high temporal resolution in response to nutrient and stress signals in yeast. The GAL1 activity in cell populations is modulated in a well-defined range of galactose concentrations, correlating with a dynamic change of histone remodeling and RNA polymerase II (RNAPII) association. This behavior is the result of a heterogeneous induction delay caused by decreasing inducer concentrations across the population. Chromatin remodeling appears to be the basis for the dynamic GAL1 expression, because mutants with impaired histone dynamics show severely truncated dose-response profiles. In contrast, the GRE2 promoter operates like a rapid off/on switch in response to increasing osmotic stress, with almost constant expression rates and exclusively temporal regulation of histone remodeling and RNAPII occupancy. The Gal3 inducer and the Hog1 mitogen-activated protein (MAP) kinase seem to determine the different dose-response strategies at the two promoters. Accordingly, GAL1 becomes highly sensitive and dose independent if previously stimulated because of residual Gal3 levels, whereas GRE2 expression diminishes upon repeated stimulation due to acquired stress resistance. Our analysis reveals important differences in the way dynamic signals create dose-sensitive gene expression outputs.
AB - Cells respond to environmental stimuli by fine-tuned regulation of gene expression. Here we investigated the dose-dependent modulation of gene expression at high temporal resolution in response to nutrient and stress signals in yeast. The GAL1 activity in cell populations is modulated in a well-defined range of galactose concentrations, correlating with a dynamic change of histone remodeling and RNA polymerase II (RNAPII) association. This behavior is the result of a heterogeneous induction delay caused by decreasing inducer concentrations across the population. Chromatin remodeling appears to be the basis for the dynamic GAL1 expression, because mutants with impaired histone dynamics show severely truncated dose-response profiles. In contrast, the GRE2 promoter operates like a rapid off/on switch in response to increasing osmotic stress, with almost constant expression rates and exclusively temporal regulation of histone remodeling and RNAPII occupancy. The Gal3 inducer and the Hog1 mitogen-activated protein (MAP) kinase seem to determine the different dose-response strategies at the two promoters. Accordingly, GAL1 becomes highly sensitive and dose independent if previously stimulated because of residual Gal3 levels, whereas GRE2 expression diminishes upon repeated stimulation due to acquired stress resistance. Our analysis reveals important differences in the way dynamic signals create dose-sensitive gene expression outputs.
KW - Galactokinase/genetics
KW - Galactose/metabolism
KW - Gene Expression Regulation, Fungal
KW - Genes, Regulator
KW - Histones/genetics
KW - Mitogen-Activated Protein Kinases/metabolism
KW - Oxidoreductases/genetics
KW - RNA Polymerase II/metabolism
KW - Saccharomyces cerevisiae/cytology
KW - Saccharomyces cerevisiae Proteins/genetics
KW - Stress, Physiological
KW - Trans-Activators/metabolism
KW - Transcription Factors/metabolism
U2 - 10.1128/MCB.00729-15
DO - 10.1128/MCB.00729-15
M3 - Journal article
C2 - 26283730
VL - 35
SP - 3669
EP - 3683
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
SN - 0270-7306
IS - 21
ER -
ID: 391636105