Standard
Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II : Advanced Characterization. / Bitzenhofer, Nora Lisa; Hilgers, Fabienne; Bosio, Gabriela N.; Torra, Joaquim; Casini, Giorgia; Beinlich, Felix R.M.; Knieps-Grünhagen, Esther; Gordeliy, Valentin; Jaeger, Karl Erich; Nonell, Santi; Krauss, Ulrich; Gensch, Thomas; Drepper, Thomas.
Methods in Molecular Biology. Vol. 2564 New York, NY : Humana Press, 2023. p. 143-183 (Methods in Molecular Biology, Vol. 2564).
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Harvard
Bitzenhofer, NL, Hilgers, F, Bosio, GN, Torra, J, Casini, G
, Beinlich, FRM, Knieps-Grünhagen, E, Gordeliy, V, Jaeger, KE, Nonell, S, Krauss, U, Gensch, T & Drepper, T 2023,
Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization. in
Methods in Molecular Biology. vol. 2564, Humana Press, New York, NY, Methods in Molecular Biology, vol. 2564, pp. 143-183.
https://doi.org/10.1007/978-1-0716-2667-2_7APA
Bitzenhofer, N. L., Hilgers, F., Bosio, G. N., Torra, J., Casini, G.
, Beinlich, F. R. M., Knieps-Grünhagen, E., Gordeliy, V., Jaeger, K. E., Nonell, S., Krauss, U., Gensch, T., & Drepper, T. (2023).
Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization. In
Methods in Molecular Biology (Vol. 2564, pp. 143-183). Humana Press. Methods in Molecular Biology Vol. 2564
https://doi.org/10.1007/978-1-0716-2667-2_7Vancouver
Bitzenhofer NL, Hilgers F, Bosio GN, Torra J, Casini G
, Beinlich FRM et al.
Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization. In Methods in Molecular Biology. Vol. 2564. New York, NY: Humana Press. 2023. p. 143-183. (Methods in Molecular Biology, Vol. 2564).
https://doi.org/10.1007/978-1-0716-2667-2_7Author
Bitzenhofer, Nora Lisa ; Hilgers, Fabienne ; Bosio, Gabriela N. ; Torra, Joaquim ; Casini, Giorgia ; Beinlich, Felix R.M. ; Knieps-Grünhagen, Esther ; Gordeliy, Valentin ; Jaeger, Karl Erich ; Nonell, Santi ; Krauss, Ulrich ; Gensch, Thomas ; Drepper, Thomas. / Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II : Advanced Characterization. Methods in Molecular Biology. Vol. 2564 New York, NY : Humana Press, 2023. pp. 143-183 (Methods in Molecular Biology, Vol. 2564).
Bibtex
@inbook{599f285cfeb1479ca0d26c2ea2063397,
title = "Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization",
abstract = "Flavin-based fluorescent proteins (FbFPs), a class of small fluorescent proteins derived from light-oxygen-voltage (LOV) domains, bind ubiquitous endogenous flavins as chromophores. Due to their unique properties, they can be used as versatile in vivo reporter proteins under aerobic and anaerobic conditions. This chapter presents methodologies for in-depth characterization of the biochemical, spectroscopic, photophysical, and photochemical properties of FbFPs.",
keywords = "Flavin, Flavin-binding fluorescent protein, Fluorescence brightness, Fluorescence lifetime, Fluorescence microscopy, Genetically encoded photosensitizer, LOV domain, Phototoxicity",
author = "Bitzenhofer, {Nora Lisa} and Fabienne Hilgers and Bosio, {Gabriela N.} and Joaquim Torra and Giorgia Casini and Beinlich, {Felix R.M.} and Esther Knieps-Gr{\"u}nhagen and Valentin Gordeliy and Jaeger, {Karl Erich} and Santi Nonell and Ulrich Krauss and Thomas Gensch and Thomas Drepper",
note = "Funding Information: N.L.B., F.H., G.B., and J.T. contributed equally to the preparation of the chapter. S.N., U.K., T.G., and T.D. are co-corresponding authors. The PS characterization was supported by grants from the Bioeconomy Science Center awarded to F.H. The scientific activities of the Bioeconomy Science Center were financially supported by the Ministry of Innovation, Science and Research of the German federal state of North Rhine-Westphalia MIWF within the frame-work of the NRW Strategieprojekt BioSC (No. 313/323-400-00213). The authors thank Ivan Gushchin for his contributions to writing and editing of this chapter. Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2667-2_7",
language = "English",
isbn = "978-1-0716-2666-5",
volume = "2564",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "143--183",
booktitle = "Methods in Molecular Biology",
address = "United States",
}
RIS
TY - CHAP
T1 - Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II
T2 - Advanced Characterization
AU - Bitzenhofer, Nora Lisa
AU - Hilgers, Fabienne
AU - Bosio, Gabriela N.
AU - Torra, Joaquim
AU - Casini, Giorgia
AU - Beinlich, Felix R.M.
AU - Knieps-Grünhagen, Esther
AU - Gordeliy, Valentin
AU - Jaeger, Karl Erich
AU - Nonell, Santi
AU - Krauss, Ulrich
AU - Gensch, Thomas
AU - Drepper, Thomas
N1 - Funding Information:
N.L.B., F.H., G.B., and J.T. contributed equally to the preparation of the chapter. S.N., U.K., T.G., and T.D. are co-corresponding authors. The PS characterization was supported by grants from the Bioeconomy Science Center awarded to F.H. The scientific activities of the Bioeconomy Science Center were financially supported by the Ministry of Innovation, Science and Research of the German federal state of North Rhine-Westphalia MIWF within the frame-work of the NRW Strategieprojekt BioSC (No. 313/323-400-00213). The authors thank Ivan Gushchin for his contributions to writing and editing of this chapter.
Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2023
Y1 - 2023
N2 - Flavin-based fluorescent proteins (FbFPs), a class of small fluorescent proteins derived from light-oxygen-voltage (LOV) domains, bind ubiquitous endogenous flavins as chromophores. Due to their unique properties, they can be used as versatile in vivo reporter proteins under aerobic and anaerobic conditions. This chapter presents methodologies for in-depth characterization of the biochemical, spectroscopic, photophysical, and photochemical properties of FbFPs.
AB - Flavin-based fluorescent proteins (FbFPs), a class of small fluorescent proteins derived from light-oxygen-voltage (LOV) domains, bind ubiquitous endogenous flavins as chromophores. Due to their unique properties, they can be used as versatile in vivo reporter proteins under aerobic and anaerobic conditions. This chapter presents methodologies for in-depth characterization of the biochemical, spectroscopic, photophysical, and photochemical properties of FbFPs.
KW - Flavin
KW - Flavin-binding fluorescent protein
KW - Fluorescence brightness
KW - Fluorescence lifetime
KW - Fluorescence microscopy
KW - Genetically encoded photosensitizer
KW - LOV domain
KW - Phototoxicity
U2 - 10.1007/978-1-0716-2667-2_7
DO - 10.1007/978-1-0716-2667-2_7
M3 - Book chapter
C2 - 36107341
AN - SCOPUS:85137880361
SN - 978-1-0716-2666-5
VL - 2564
T3 - Methods in Molecular Biology
SP - 143
EP - 183
BT - Methods in Molecular Biology
PB - Humana Press
CY - New York, NY
ER -
