Determination of the adhesive capability of canine polymorphonuclear neutrophil granulocytes using a fluorometric microtiter plate cellular adhesion assay

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Determination of the adhesive capability of canine polymorphonuclear neutrophil granulocytes using a fluorometric microtiter plate cellular adhesion assay. / Petersen, Thomas Kongstad; Væver Bysted, Britta; Jensen, Asger Lundorff.

In: Veterinary Immunology and Immunopathology, Vol. 68, No. 2-4, 05.1999, p. 283-291.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Petersen, TK, Væver Bysted, B & Jensen, AL 1999, 'Determination of the adhesive capability of canine polymorphonuclear neutrophil granulocytes using a fluorometric microtiter plate cellular adhesion assay', Veterinary Immunology and Immunopathology, vol. 68, no. 2-4, pp. 283-291. https://doi.org/10.1016/S0165-2427(99)00016-1

APA

Petersen, T. K., Væver Bysted, B., & Jensen, A. L. (1999). Determination of the adhesive capability of canine polymorphonuclear neutrophil granulocytes using a fluorometric microtiter plate cellular adhesion assay. Veterinary Immunology and Immunopathology, 68(2-4), 283-291. https://doi.org/10.1016/S0165-2427(99)00016-1

Vancouver

Petersen TK, Væver Bysted B, Jensen AL. Determination of the adhesive capability of canine polymorphonuclear neutrophil granulocytes using a fluorometric microtiter plate cellular adhesion assay. Veterinary Immunology and Immunopathology. 1999 May;68(2-4):283-291. https://doi.org/10.1016/S0165-2427(99)00016-1

Author

Petersen, Thomas Kongstad ; Væver Bysted, Britta ; Jensen, Asger Lundorff. / Determination of the adhesive capability of canine polymorphonuclear neutrophil granulocytes using a fluorometric microtiter plate cellular adhesion assay. In: Veterinary Immunology and Immunopathology. 1999 ; Vol. 68, No. 2-4. pp. 283-291.

Bibtex

@article{14afae5adea24d928322c2c6cfae5b7e,
title = "Determination of the adhesive capability of canine polymorphonuclear neutrophil granulocytes using a fluorometric microtiter plate cellular adhesion assay",
abstract = "A rapid fluorometric cellular adhesion assay in microtiter plates was evaluated for canine polymorphonuclear neutrophil granulocytes (PMN) using the fluorescent indicator calcein acetoxymethyl ester (Calcein AM). Optimum adhesive responsiveness occurred in bovine serum albumin (BSA)-coated plates after 30 min incubation at 37°C with 5 x 105 PMN in 100 μl per well using phorbol myristate acetate (PMA, 100 ng/ml) as stimulant. The adhesive responsiveness for 15 laboratory beagle dogs was determined and the mean percentage of adherence for unstimulated (2.9%) and stimulated (74.3%) PMN and corresponding 95% confidence intervals was determined. The mean intra- assay coefficient of variation (CV(intra)) for unstimulated and stimulated adhesion in the assay was: CV(intra) = 12.9% and CV(intra) = 4.1%, respectively. The mean inter-assay coefficient of variation (CV(inter)) for unstimulated and stimulated adhesion, respectively, in the assay was: CV(inter) = 22.9% and CV(inter) = 5.6%. The assay was highly reproducible and labelling with Calcein AM neither reduced PMN viability nor activated the PMN.",
keywords = "Adhesion-assay, Calcein AM, Canine, Fluorescent, Microtiter plates, Neutrophils",
author = "Petersen, {Thomas Kongstad} and {V{\ae}ver Bysted}, Britta and Jensen, {Asger Lundorff}",
year = "1999",
month = may,
doi = "10.1016/S0165-2427(99)00016-1",
language = "English",
volume = "68",
pages = "283--291",
journal = "Veterinary Immunology and Immunopathology",
issn = "0165-2427",
publisher = "Elsevier",
number = "2-4",

}

RIS

TY - JOUR

T1 - Determination of the adhesive capability of canine polymorphonuclear neutrophil granulocytes using a fluorometric microtiter plate cellular adhesion assay

AU - Petersen, Thomas Kongstad

AU - Væver Bysted, Britta

AU - Jensen, Asger Lundorff

PY - 1999/5

Y1 - 1999/5

N2 - A rapid fluorometric cellular adhesion assay in microtiter plates was evaluated for canine polymorphonuclear neutrophil granulocytes (PMN) using the fluorescent indicator calcein acetoxymethyl ester (Calcein AM). Optimum adhesive responsiveness occurred in bovine serum albumin (BSA)-coated plates after 30 min incubation at 37°C with 5 x 105 PMN in 100 μl per well using phorbol myristate acetate (PMA, 100 ng/ml) as stimulant. The adhesive responsiveness for 15 laboratory beagle dogs was determined and the mean percentage of adherence for unstimulated (2.9%) and stimulated (74.3%) PMN and corresponding 95% confidence intervals was determined. The mean intra- assay coefficient of variation (CV(intra)) for unstimulated and stimulated adhesion in the assay was: CV(intra) = 12.9% and CV(intra) = 4.1%, respectively. The mean inter-assay coefficient of variation (CV(inter)) for unstimulated and stimulated adhesion, respectively, in the assay was: CV(inter) = 22.9% and CV(inter) = 5.6%. The assay was highly reproducible and labelling with Calcein AM neither reduced PMN viability nor activated the PMN.

AB - A rapid fluorometric cellular adhesion assay in microtiter plates was evaluated for canine polymorphonuclear neutrophil granulocytes (PMN) using the fluorescent indicator calcein acetoxymethyl ester (Calcein AM). Optimum adhesive responsiveness occurred in bovine serum albumin (BSA)-coated plates after 30 min incubation at 37°C with 5 x 105 PMN in 100 μl per well using phorbol myristate acetate (PMA, 100 ng/ml) as stimulant. The adhesive responsiveness for 15 laboratory beagle dogs was determined and the mean percentage of adherence for unstimulated (2.9%) and stimulated (74.3%) PMN and corresponding 95% confidence intervals was determined. The mean intra- assay coefficient of variation (CV(intra)) for unstimulated and stimulated adhesion in the assay was: CV(intra) = 12.9% and CV(intra) = 4.1%, respectively. The mean inter-assay coefficient of variation (CV(inter)) for unstimulated and stimulated adhesion, respectively, in the assay was: CV(inter) = 22.9% and CV(inter) = 5.6%. The assay was highly reproducible and labelling with Calcein AM neither reduced PMN viability nor activated the PMN.

KW - Adhesion-assay

KW - Calcein AM

KW - Canine

KW - Fluorescent

KW - Microtiter plates

KW - Neutrophils

UR - http://www.scopus.com/inward/record.url?scp=0344848801&partnerID=8YFLogxK

U2 - 10.1016/S0165-2427(99)00016-1

DO - 10.1016/S0165-2427(99)00016-1

M3 - Journal article

C2 - 10438326

AN - SCOPUS:0344848801

VL - 68

SP - 283

EP - 291

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

SN - 0165-2427

IS - 2-4

ER -

ID: 255558580