Detection of the biofilm component polysaccharide intercellular adhesin in Staphylococcus aureus infected cow udders
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Detection of the biofilm component polysaccharide intercellular adhesin in Staphylococcus aureus infected cow udders. / Schönborn, Sarah; Krömker, Volker.
In: Veterinary Microbiology, Vol. 196, 30.11.2016, p. 126-128.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Detection of the biofilm component polysaccharide intercellular adhesin in Staphylococcus aureus infected cow udders
AU - Schönborn, Sarah
AU - Krömker, Volker
N1 - Copyright © 2016 Elsevier B.V. All rights reserved.
PY - 2016/11/30
Y1 - 2016/11/30
N2 - Biofilms are communities of microorganisms embedded in a self-produced extracellular matrix made up of polymeric substances. They reduce the effects of antibiotics and allow the microorganisms to evade the innate immune system. This can lead to persistent or recurrent infections. In dairy cow herds, mastitis is a serious problem. The present study aimed to investigate the occurrence of biofilms in the udders of dairy cows infected with Staphylococcus (S.) aureus, because biofilms may affect the response to treatment of bovine mastitis. Immunofluorescence staining of polysaccharide intercellular adhesin (PIA), a component of S. aureus biofilms, was carried out based on swabs taken from different areas of S. aureus infected udders. We were able to demonstrate the presence of PIA in S. aureus infected bovine udders. However, the applied method is invasive and therefore only really suitable for scientific research and not for clinical diagnosis.
AB - Biofilms are communities of microorganisms embedded in a self-produced extracellular matrix made up of polymeric substances. They reduce the effects of antibiotics and allow the microorganisms to evade the innate immune system. This can lead to persistent or recurrent infections. In dairy cow herds, mastitis is a serious problem. The present study aimed to investigate the occurrence of biofilms in the udders of dairy cows infected with Staphylococcus (S.) aureus, because biofilms may affect the response to treatment of bovine mastitis. Immunofluorescence staining of polysaccharide intercellular adhesin (PIA), a component of S. aureus biofilms, was carried out based on swabs taken from different areas of S. aureus infected udders. We were able to demonstrate the presence of PIA in S. aureus infected bovine udders. However, the applied method is invasive and therefore only really suitable for scientific research and not for clinical diagnosis.
KW - Animals
KW - Anti-Bacterial Agents/pharmacology
KW - Biofilms/growth & development
KW - Cattle
KW - Female
KW - Mammary Glands, Animal/microbiology
KW - Mastitis, Bovine/microbiology
KW - Polysaccharides, Bacterial/genetics
KW - Staphylococcal Infections/microbiology
KW - Staphylococcus aureus/drug effects
KW - beta-Glucans
U2 - 10.1016/j.vetmic.2016.10.023
DO - 10.1016/j.vetmic.2016.10.023
M3 - Journal article
C2 - 27939148
VL - 196
SP - 126
EP - 128
JO - Veterinary Microbiology
JF - Veterinary Microbiology
SN - 0378-1135
ER -
ID: 237051528