Detection of SARS-CoV-2 in exhaled breath from non-hospitalized COVID-19-infected individuals
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Detection of SARS-CoV-2 in exhaled breath from non-hospitalized COVID-19-infected individuals. / Leding, Cæcilie; Skov, Julia; Uhrbrand, Katrine; Lisby, Jan Gorm; Hansen, Katrine Pedersbæk; Benfield, Thomas; Duncan, Louise Katrine.
In: Scientific Reports, Vol. 12, 11151, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Detection of SARS-CoV-2 in exhaled breath from non-hospitalized COVID-19-infected individuals
AU - Leding, Cæcilie
AU - Skov, Julia
AU - Uhrbrand, Katrine
AU - Lisby, Jan Gorm
AU - Hansen, Katrine Pedersbæk
AU - Benfield, Thomas
AU - Duncan, Louise Katrine
N1 - Publisher Copyright: © 2022, The Author(s).
PY - 2022
Y1 - 2022
N2 - The diagnosis of COVID-19 is based on detection of SARS-CoV-2 in oro-/nasopharyngel swabs, but due to discomfort and minor risk during the swab procedure, detection of SARS-CoV-2 has been investigated in other biological matrixes. In this proof-of-concept study, individuals with confirmed SARS-CoV-2 infection performed a daily air sample for five days. Air samples were obtained through a non-invasive electrostatic air sampler. Detection of SARS-CoV-2 RNA was determined with qRT-PCR. The association of positive samples with different exposures was evaluated through mixed-effect models. We obtained 665 air samples from 111 included participants with confirmed SARS-CoV-2 infection. Overall, 52 individuals (46.8%) had at least one positive air sample, and 129 (19.4%) air samples were positive for SARS-CoV-2. Participants with symptoms or a symptom duration ≤ four days had significantly higher odds of having a positive air sample. Cycle threshold values were significantly lower in samples obtained ≤ 4 days from symptom onset. Neither variant of SARS-CoV-2 nor method of air sampling were associated with a positive air sample. We demonstrate that SARS-CoV-2 is detectable in human breath by electrostatic air sampling with the highest detection rate closest to symptom onset. We suggest further evaluation of the air sampling technique to increase sensitivity.
AB - The diagnosis of COVID-19 is based on detection of SARS-CoV-2 in oro-/nasopharyngel swabs, but due to discomfort and minor risk during the swab procedure, detection of SARS-CoV-2 has been investigated in other biological matrixes. In this proof-of-concept study, individuals with confirmed SARS-CoV-2 infection performed a daily air sample for five days. Air samples were obtained through a non-invasive electrostatic air sampler. Detection of SARS-CoV-2 RNA was determined with qRT-PCR. The association of positive samples with different exposures was evaluated through mixed-effect models. We obtained 665 air samples from 111 included participants with confirmed SARS-CoV-2 infection. Overall, 52 individuals (46.8%) had at least one positive air sample, and 129 (19.4%) air samples were positive for SARS-CoV-2. Participants with symptoms or a symptom duration ≤ four days had significantly higher odds of having a positive air sample. Cycle threshold values were significantly lower in samples obtained ≤ 4 days from symptom onset. Neither variant of SARS-CoV-2 nor method of air sampling were associated with a positive air sample. We demonstrate that SARS-CoV-2 is detectable in human breath by electrostatic air sampling with the highest detection rate closest to symptom onset. We suggest further evaluation of the air sampling technique to increase sensitivity.
U2 - 10.1038/s41598-022-15243-1
DO - 10.1038/s41598-022-15243-1
M3 - Journal article
C2 - 35778461
AN - SCOPUS:85133289736
VL - 12
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 11151
ER -
ID: 319406216