Detection of Lawsonia intracellularis, Serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes, Salmonella enterica, and haemolytic Escherichia coli from swine herds with and without diarrhoea among growing pigs

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Detection of Lawsonia intracellularis, Serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes, Salmonella enterica, and haemolytic Escherichia coli from swine herds with and without diarrhoea among growing pigs. / Møller, Kristian; Jensen, Tim K.; Jorsal, Sven E.; Leser, Thomas D.; Carstensen, Bendix.

In: Veterinary Microbiology, Vol. 62, No. 1, 30.04.1998, p. 59-72.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Møller, K, Jensen, TK, Jorsal, SE, Leser, TD & Carstensen, B 1998, 'Detection of Lawsonia intracellularis, Serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes, Salmonella enterica, and haemolytic Escherichia coli from swine herds with and without diarrhoea among growing pigs', Veterinary Microbiology, vol. 62, no. 1, pp. 59-72. https://doi.org/10.1016/S0378-1135(98)00199-0

APA

Møller, K., Jensen, T. K., Jorsal, S. E., Leser, T. D., & Carstensen, B. (1998). Detection of Lawsonia intracellularis, Serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes, Salmonella enterica, and haemolytic Escherichia coli from swine herds with and without diarrhoea among growing pigs. Veterinary Microbiology, 62(1), 59-72. https://doi.org/10.1016/S0378-1135(98)00199-0

Vancouver

Møller K, Jensen TK, Jorsal SE, Leser TD, Carstensen B. Detection of Lawsonia intracellularis, Serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes, Salmonella enterica, and haemolytic Escherichia coli from swine herds with and without diarrhoea among growing pigs. Veterinary Microbiology. 1998 Apr 30;62(1):59-72. https://doi.org/10.1016/S0378-1135(98)00199-0

Author

Møller, Kristian ; Jensen, Tim K. ; Jorsal, Sven E. ; Leser, Thomas D. ; Carstensen, Bendix. / Detection of Lawsonia intracellularis, Serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes, Salmonella enterica, and haemolytic Escherichia coli from swine herds with and without diarrhoea among growing pigs. In: Veterinary Microbiology. 1998 ; Vol. 62, No. 1. pp. 59-72.

Bibtex

@article{54cdcb8e479a4545b801211206d2b1c0,
title = "Detection of Lawsonia intracellularis, Serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes, Salmonella enterica, and haemolytic Escherichia coli from swine herds with and without diarrhoea among growing pigs",
abstract = "A polymerase chain reaction (PCR) was optimized to detect Lawsonia intracellularis in faeces from naturally infected pigs. By combining a boiling procedure to extract DNA and a nested PCR procedure, a detection limit at 2x 102 bacterial cells per gram of faeces was achieved. The optimized PCR was used together with conventional culture techniques to detect Serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes (WBHIS), Salmonella enterica, and haemolytic Escherichia coli, in a case control study to examine selected risk factors for the development of diarrhoea in growing pigs. Herds with diarrhoea were selected as cases and randomly chosen herds without diarrhoea were chosen as controls. Infection with L. intracellularis significantly enhanced the chance of diarrhoea. S. hyodysenteriae, WBHIS group IV (Serpulina pilosicoli), and S. enterica were isolated only from case herds which indicate that these species may influence the development of diarrhoea. In addition, herd-type had a significant impact, that is specific pathogen-free herds showed an odds ratio at 0.2 relative to conventional herds for the development of diarrhoea.",
keywords = "Case control study, Pig-bacteria, Proliferative enteropathy, Serpulina sp.",
author = "Kristian M{\o}ller and Jensen, {Tim K.} and Jorsal, {Sven E.} and Leser, {Thomas D.} and Bendix Carstensen",
note = "Funding Information: This work was supported by the Research Secretariat of the Ministry of Food, Agriculture and Fisheries, project number SUN93-3. The authors thank P.H. Andersen, L.T.M. Nguyen and S.M. Jensen for excellent technical assistance. ",
year = "1998",
month = apr,
day = "30",
doi = "10.1016/S0378-1135(98)00199-0",
language = "English",
volume = "62",
pages = "59--72",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Detection of Lawsonia intracellularis, Serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes, Salmonella enterica, and haemolytic Escherichia coli from swine herds with and without diarrhoea among growing pigs

AU - Møller, Kristian

AU - Jensen, Tim K.

AU - Jorsal, Sven E.

AU - Leser, Thomas D.

AU - Carstensen, Bendix

N1 - Funding Information: This work was supported by the Research Secretariat of the Ministry of Food, Agriculture and Fisheries, project number SUN93-3. The authors thank P.H. Andersen, L.T.M. Nguyen and S.M. Jensen for excellent technical assistance.

PY - 1998/4/30

Y1 - 1998/4/30

N2 - A polymerase chain reaction (PCR) was optimized to detect Lawsonia intracellularis in faeces from naturally infected pigs. By combining a boiling procedure to extract DNA and a nested PCR procedure, a detection limit at 2x 102 bacterial cells per gram of faeces was achieved. The optimized PCR was used together with conventional culture techniques to detect Serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes (WBHIS), Salmonella enterica, and haemolytic Escherichia coli, in a case control study to examine selected risk factors for the development of diarrhoea in growing pigs. Herds with diarrhoea were selected as cases and randomly chosen herds without diarrhoea were chosen as controls. Infection with L. intracellularis significantly enhanced the chance of diarrhoea. S. hyodysenteriae, WBHIS group IV (Serpulina pilosicoli), and S. enterica were isolated only from case herds which indicate that these species may influence the development of diarrhoea. In addition, herd-type had a significant impact, that is specific pathogen-free herds showed an odds ratio at 0.2 relative to conventional herds for the development of diarrhoea.

AB - A polymerase chain reaction (PCR) was optimized to detect Lawsonia intracellularis in faeces from naturally infected pigs. By combining a boiling procedure to extract DNA and a nested PCR procedure, a detection limit at 2x 102 bacterial cells per gram of faeces was achieved. The optimized PCR was used together with conventional culture techniques to detect Serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes (WBHIS), Salmonella enterica, and haemolytic Escherichia coli, in a case control study to examine selected risk factors for the development of diarrhoea in growing pigs. Herds with diarrhoea were selected as cases and randomly chosen herds without diarrhoea were chosen as controls. Infection with L. intracellularis significantly enhanced the chance of diarrhoea. S. hyodysenteriae, WBHIS group IV (Serpulina pilosicoli), and S. enterica were isolated only from case herds which indicate that these species may influence the development of diarrhoea. In addition, herd-type had a significant impact, that is specific pathogen-free herds showed an odds ratio at 0.2 relative to conventional herds for the development of diarrhoea.

KW - Case control study

KW - Pig-bacteria

KW - Proliferative enteropathy

KW - Serpulina sp.

UR - http://www.scopus.com/inward/record.url?scp=0032580263&partnerID=8YFLogxK

U2 - 10.1016/S0378-1135(98)00199-0

DO - 10.1016/S0378-1135(98)00199-0

M3 - Journal article

C2 - 9659692

AN - SCOPUS:0032580263

VL - 62

SP - 59

EP - 72

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 1

ER -

ID: 339895933