Detection of Bacteria by Fluorescence in Situ Hybridization in Culture-Negative Soft Tissue Filler Lesions
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Detection of Bacteria by Fluorescence in Situ Hybridization in Culture-Negative Soft Tissue Filler Lesions. / Bjarnsholt, Thomas; Tolker-Nielsen, Tim; Givskov, Michael; Janssen, Martin; Christensen, Lise Hanne.
In: Dermatologic Surgery, Vol. 35 Suppl 2, 2009, p. 1620-1624.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Detection of Bacteria by Fluorescence in Situ Hybridization in Culture-Negative Soft Tissue Filler Lesions
AU - Bjarnsholt, Thomas
AU - Tolker-Nielsen, Tim
AU - Givskov, Michael
AU - Janssen, Martin
AU - Christensen, Lise Hanne
PY - 2009
Y1 - 2009
N2 - BACKGROUND Adverse reactions to polyacrylamide gel occur as swellings or nodules, and controversy exists whether these are due to bacterial infection or an autoimmune reaction to the filler. OBJECTIVES Biopsies from culture-negative long-lasting nodules after injection with different types of polyacrylamide gel were examined with a combination of Gram stain and fluorescence in situ hybridization. RESULTS Bacteria were detected in biopsies from seven of eight patients. They inhabited gel and intervening tissue and tended to lie in aggregates. CONCLUSION This study supports the assumption that infection with bacteria in aggregates causes culture-negative late adverse reactions to polyacrylamide gel, suggesting a biofilm environment. The authors have indicated no significant interest with commercial supporters.
AB - BACKGROUND Adverse reactions to polyacrylamide gel occur as swellings or nodules, and controversy exists whether these are due to bacterial infection or an autoimmune reaction to the filler. OBJECTIVES Biopsies from culture-negative long-lasting nodules after injection with different types of polyacrylamide gel were examined with a combination of Gram stain and fluorescence in situ hybridization. RESULTS Bacteria were detected in biopsies from seven of eight patients. They inhabited gel and intervening tissue and tended to lie in aggregates. CONCLUSION This study supports the assumption that infection with bacteria in aggregates causes culture-negative late adverse reactions to polyacrylamide gel, suggesting a biofilm environment. The authors have indicated no significant interest with commercial supporters.
U2 - 10.1111/j.1524-4725.2009.01313.x
DO - 10.1111/j.1524-4725.2009.01313.x
M3 - Journal article
C2 - 19709133
VL - 35 Suppl 2
SP - 1620
EP - 1624
JO - Dermatologic Surgery
JF - Dermatologic Surgery
SN - 1076-0512
ER -
ID: 14940726