Detection of antibodies to human immunodeficiency virus (HIV) in eluates from whole blood impregnated filter paper discs
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Detection of antibodies to human immunodeficiency virus (HIV) in eluates from whole blood impregnated filter paper discs. / Lindhardt, B O; Bygbjerg, Ib Christian; Ulrich, K; Petersen, H D; Lausen, I; Frederiksen, B.
In: Journal of Virological Methods, Vol. 18, No. 1, 01.10.1987, p. 73-7.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Detection of antibodies to human immunodeficiency virus (HIV) in eluates from whole blood impregnated filter paper discs
AU - Lindhardt, B O
AU - Bygbjerg, Ib Christian
AU - Ulrich, K
AU - Petersen, H D
AU - Lausen, I
AU - Frederiksen, B
PY - 1987/10/1
Y1 - 1987/10/1
N2 - A method for elution of HIV antibodies from whole blood or serum impregnated filter paper discs was developed. The results from testing of 73 eluates in an enzyme linked immunosorbent assay and the immunoblotting test agreed with the results obtained by ordinary serum testing. Significant loss of antibody activity was not observed, neither in the eluates after storage for 1 mth at -20 degrees C nor in the filter paper discs after storage for 3 mths at +4 degrees C. This technique may be useful in facilitating sample collection and transportation, particularly in remote areas of the world.
AB - A method for elution of HIV antibodies from whole blood or serum impregnated filter paper discs was developed. The results from testing of 73 eluates in an enzyme linked immunosorbent assay and the immunoblotting test agreed with the results obtained by ordinary serum testing. Significant loss of antibody activity was not observed, neither in the eluates after storage for 1 mth at -20 degrees C nor in the filter paper discs after storage for 3 mths at +4 degrees C. This technique may be useful in facilitating sample collection and transportation, particularly in remote areas of the world.
KW - Africa, Central
KW - Africa, Eastern
KW - Antibodies, Viral
KW - Denmark
KW - Enzyme-Linked Immunosorbent Assay
KW - HIV
KW - HIV Antibodies
KW - HIV Seropositivity
KW - Humans
KW - Paper
M3 - Journal article
C2 - 3480292
VL - 18
SP - 73
EP - 77
JO - Journal of Virological Methods
JF - Journal of Virological Methods
SN - 0166-0934
IS - 1
ER -
ID: 33891478