Dendritic calcium activity precedes inspiratory bursts in preBotzinger complex neurons
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Dendritic calcium activity precedes inspiratory bursts in preBotzinger complex neurons. / Del Negro, Christopher A; Hayes, John A; Rekling, Jens C.
In: Journal of Neuroscience, Vol. 31, No. 3, 19.01.2011, p. 1017-22.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Dendritic calcium activity precedes inspiratory bursts in preBotzinger complex neurons
AU - Del Negro, Christopher A
AU - Hayes, John A
AU - Rekling, Jens C
PY - 2011/1/19
Y1 - 2011/1/19
N2 - Medullary interneurons of the preBötzinger complex assemble excitatory networks that produce inspiratory-related neural rhythms, but the importance of somatodendritic conductances in rhythm generation is still incompletely understood. Synaptic input may cause Ca(2+) accumulation postsynaptically to evoke a Ca(2+)-activated inward current that contributes to inspiratory burst generation. We measured Ca(2+) transients by two-photon imaging dendrites while recording neuronal somata electrophysiologically. Dendritic Ca(2+) accumulation frequently precedes inspiratory bursts, particularly at recording sites 50-300 µm distal from the soma. Preinspiratory Ca(2+) transients occur in hotspots, not ubiquitously, in dendrites. Ca(2+) activity propagates orthodromically toward the soma (and antidromically to more distal regions of the dendrite) at rapid rates (300-700 µm/s). These high propagation rates suggest that dendritic Ca(2+) activates an inward current to electrotonically depolarize the soma, rather than propagate as a regenerative Ca(2+) wave. These data provide new evidence that respiratory rhythmogenesis may depend on dendritic burst-generating conductances activated in the context of network activity.
AB - Medullary interneurons of the preBötzinger complex assemble excitatory networks that produce inspiratory-related neural rhythms, but the importance of somatodendritic conductances in rhythm generation is still incompletely understood. Synaptic input may cause Ca(2+) accumulation postsynaptically to evoke a Ca(2+)-activated inward current that contributes to inspiratory burst generation. We measured Ca(2+) transients by two-photon imaging dendrites while recording neuronal somata electrophysiologically. Dendritic Ca(2+) accumulation frequently precedes inspiratory bursts, particularly at recording sites 50-300 µm distal from the soma. Preinspiratory Ca(2+) transients occur in hotspots, not ubiquitously, in dendrites. Ca(2+) activity propagates orthodromically toward the soma (and antidromically to more distal regions of the dendrite) at rapid rates (300-700 µm/s). These high propagation rates suggest that dendritic Ca(2+) activates an inward current to electrotonically depolarize the soma, rather than propagate as a regenerative Ca(2+) wave. These data provide new evidence that respiratory rhythmogenesis may depend on dendritic burst-generating conductances activated in the context of network activity.
KW - Action Potentials
KW - Animals
KW - Calcium
KW - Dendrites
KW - Electrophysiology
KW - Mice
KW - Neurons
KW - Respiratory Center
U2 - 10.1523/JNEUROSCI.4731-10.2011
DO - 10.1523/JNEUROSCI.4731-10.2011
M3 - Journal article
C2 - 21248126
VL - 31
SP - 1017
EP - 1022
JO - The Journal of neuroscience : the official journal of the Society for Neuroscience
JF - The Journal of neuroscience : the official journal of the Society for Neuroscience
SN - 0270-6474
IS - 3
ER -
ID: 33754560