Cytotoxicity of Prymnesium parvum extracts and prymnesin analogs on epithelial fish gill cells RTgill‑W1 and the human colon cell line HCEC‑1CT: [Inkl. Correction]
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Cytotoxicity of Prymnesium parvum extracts and prymnesin analogs on epithelial fish gill cells RTgill‑W1 and the human colon cell line HCEC‑1CT : [Inkl. Correction]. / Varga, Elisabeth; Prause, Hélène‑Christine; Riepl, Matthias; Hochmayr, Nadine; Berk, Deniz; Attakpah, Eva; Kiss, Endre; Medic, Nikola; Del Favero, Giorgia; Larsen, Thomas Ostenfeld; Hansen, Per Juel; Marko, Doris.
In: Archives of Toxicology, Vol. 98, 2024, p. 999–1014.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Cytotoxicity of Prymnesium parvum extracts and prymnesin analogs on epithelial fish gill cells RTgill‑W1 and the human colon cell line HCEC‑1CT
T2 - [Inkl. Correction]
AU - Varga, Elisabeth
AU - Prause, Hélène‑Christine
AU - Riepl, Matthias
AU - Hochmayr, Nadine
AU - Berk, Deniz
AU - Attakpah, Eva
AU - Kiss, Endre
AU - Medic, Nikola
AU - Del Favero, Giorgia
AU - Larsen, Thomas Ostenfeld
AU - Hansen, Per Juel
AU - Marko, Doris
N1 - Correction: https://doi.org/10.1007/s00204-024-03716-3
PY - 2024
Y1 - 2024
N2 - Harmful algal blooms kill fish populations worldwide, as exemplified by the haptophyte microalga Prymnesium parvum. The suspected causative agents are prymnesins, categorized as A-, B-, and C-types based on backbone carbon atoms. Impacts of P. parvum extracts and purified prymnesins were tested on the epithelial rainbow trout fish gill cell line RTgill-W1 and on the human colon epithelial cells HCEC-1CT. Cytotoxic potencies ranked A > C > B-type with concentrations spanning from low (A- and C-type) to middle (B-type) nM ranges. Although RTgill-W1 cells were about twofold more sensitive than HCEC-1CT, the cytotoxicity of prymnesins is not limited to fish gills. Both cell lines responded rapidly to prymnesins; with EC50 values for B-types in RTgill-W1 cells of 110 ± 11 nM and 41.5 ± 0.6 nM after incubations times of 3 and 24 h. Results of fluorescence imaging and measured lytic effects suggest plasma membrane interactions. Postulating an osmotic imbalance as mechanisms of toxicity, incubations with prymnesins in media lacking either Cl−, Na+, or Ca2+ were performed. Cl− removal reduced morphometric rearrangements observed in RTgill-W1 and cytotoxicity in HCEC-1CT cells. Ca2+-free medium in RTgill-W1 cells exacerbated effects on the cell nuclei. Prymnesin composition of different P. parvum strains showed that analog composition within one type scarcely influenced the cytotoxic potential, while analog type potentially dictate potency. Overall, A-type prymnesins were the most potent ones in both cell lines followed by the C-types, and lastly B-types. Disturbance of Ca2+ and Cl− ionoregulation may be integral to prymnesin toxicity.
AB - Harmful algal blooms kill fish populations worldwide, as exemplified by the haptophyte microalga Prymnesium parvum. The suspected causative agents are prymnesins, categorized as A-, B-, and C-types based on backbone carbon atoms. Impacts of P. parvum extracts and purified prymnesins were tested on the epithelial rainbow trout fish gill cell line RTgill-W1 and on the human colon epithelial cells HCEC-1CT. Cytotoxic potencies ranked A > C > B-type with concentrations spanning from low (A- and C-type) to middle (B-type) nM ranges. Although RTgill-W1 cells were about twofold more sensitive than HCEC-1CT, the cytotoxicity of prymnesins is not limited to fish gills. Both cell lines responded rapidly to prymnesins; with EC50 values for B-types in RTgill-W1 cells of 110 ± 11 nM and 41.5 ± 0.6 nM after incubations times of 3 and 24 h. Results of fluorescence imaging and measured lytic effects suggest plasma membrane interactions. Postulating an osmotic imbalance as mechanisms of toxicity, incubations with prymnesins in media lacking either Cl−, Na+, or Ca2+ were performed. Cl− removal reduced morphometric rearrangements observed in RTgill-W1 and cytotoxicity in HCEC-1CT cells. Ca2+-free medium in RTgill-W1 cells exacerbated effects on the cell nuclei. Prymnesin composition of different P. parvum strains showed that analog composition within one type scarcely influenced the cytotoxic potential, while analog type potentially dictate potency. Overall, A-type prymnesins were the most potent ones in both cell lines followed by the C-types, and lastly B-types. Disturbance of Ca2+ and Cl− ionoregulation may be integral to prymnesin toxicity.
U2 - 10.1007/s00204-023-03663-5
DO - 10.1007/s00204-023-03663-5
M3 - Journal article
C2 - 38212450
VL - 98
SP - 999
EP - 1014
JO - Archives of Toxicology
JF - Archives of Toxicology
SN - 0340-5761
ER -
ID: 379591711