Cranberry (Vaccinium macrocarpon) oligosaccharides decrease biofilm formation by uropathogenic Escherichia coli
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Cranberry (Vaccinium macrocarpon) oligosaccharides decrease biofilm formation by uropathogenic Escherichia coli. / Sun, Jiadong; Marais, Jannie P J; Khoo, Christina; LaPlante, Kerry; Vejborg, Rebecca M; Givskov, Michael; Tolker-Nielsen, Tim; Seeram, Navindra P; Rowley, David C.
In: Journal of Functional Foods, Vol. 17, 08.2015, p. 235-242.Research output: Contribution to journal › Letter › Research › peer-review
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TY - JOUR
T1 - Cranberry (Vaccinium macrocarpon) oligosaccharides decrease biofilm formation by uropathogenic Escherichia coli
AU - Sun, Jiadong
AU - Marais, Jannie P J
AU - Khoo, Christina
AU - LaPlante, Kerry
AU - Vejborg, Rebecca M
AU - Givskov, Michael
AU - Tolker-Nielsen, Tim
AU - Seeram, Navindra P
AU - Rowley, David C
PY - 2015/8
Y1 - 2015/8
N2 - The preventive effects of the American cranberry (Vaccinium macrocarpon) against urinary tract infections are supported by extensive studies which have primarily focused on its phenolic constituents. Herein, a phenolic-free carbohydrate fraction (designated cranf1b-F2) was purified from cranberry fruit using ion exchange and size exclusion chromatography. MALDI-TOF-MS analysis revealed that the cranf1b-F2 constituents are predominantly oligosaccharides possessing various degrees of polymerisation and further structural analysis (by GC-MS and NMR) revealed mainly xyloglucan and arabinan residues. In antimicrobial assays, cranf1b-F2 (at 1.25 mg/mL concentration) reduced biofilm production by the uropathogenic Escherichia coli CFT073 strain by over 50% but did not inhibit bacterial growth. Cranf1b-F2 (ranging from 0.625 - 10 mg/mL) also inhibited biofilm formation of the non-pathogenic E. coli MG1655 strain up to 60% in a concentration-dependent manner. These results suggest that cranberry oligosaccharides, in addition to its phenolic constituents, may play a role in its preventive effects against urinary tract infections.
AB - The preventive effects of the American cranberry (Vaccinium macrocarpon) against urinary tract infections are supported by extensive studies which have primarily focused on its phenolic constituents. Herein, a phenolic-free carbohydrate fraction (designated cranf1b-F2) was purified from cranberry fruit using ion exchange and size exclusion chromatography. MALDI-TOF-MS analysis revealed that the cranf1b-F2 constituents are predominantly oligosaccharides possessing various degrees of polymerisation and further structural analysis (by GC-MS and NMR) revealed mainly xyloglucan and arabinan residues. In antimicrobial assays, cranf1b-F2 (at 1.25 mg/mL concentration) reduced biofilm production by the uropathogenic Escherichia coli CFT073 strain by over 50% but did not inhibit bacterial growth. Cranf1b-F2 (ranging from 0.625 - 10 mg/mL) also inhibited biofilm formation of the non-pathogenic E. coli MG1655 strain up to 60% in a concentration-dependent manner. These results suggest that cranberry oligosaccharides, in addition to its phenolic constituents, may play a role in its preventive effects against urinary tract infections.
U2 - 10.1016/j.jff.2015.05.016
DO - 10.1016/j.jff.2015.05.016
M3 - Letter
C2 - 26613004
VL - 17
SP - 235
EP - 242
JO - Journal of Functional Foods
JF - Journal of Functional Foods
SN - 1756-4646
ER -
ID: 152934000