Copy mutants of plasmid R1: effects of base pair substitutions in the copA gene on the replication control system
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Copy mutants of plasmid R1 : effects of base pair substitutions in the copA gene on the replication control system. / Givskov, M; Molin, Søren.
In: Molecular and General Genetics MGG, Vol. 194, No. 1-2, 1984, p. 286-92.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Copy mutants of plasmid R1
T2 - effects of base pair substitutions in the copA gene on the replication control system
AU - Givskov, M
AU - Molin, Søren
PY - 1984
Y1 - 1984
N2 - Five different copA copy mutants of plasmid R1 have been identified by nucleotide sequencing. Independent measurements of the activities of the mutant inhibitor RNA and of the mutant target properties were carried out using several different methods. Correlation of these measurements with the location of th nucleotide substitutions resulted in the following conclusions: (1) The copy number of plasmid R1 is controlled primarily by interaction between the CopA RNA molecule and its target, the RepA mRNA. (2) The binding of th inhibitor to its target is based on nucleotide interactions within two complementary sequences of ten nucleotides and dependent on the secondary structure of the active site. (3) The secondary structure of both the CopA target and the CopA RNA is a stem-loop structure. Mutations in the loop region interfere with binding affinity between inhibitor and target, whereas mutations in the upper stem mainly interfere with secondary structure. Mutations in the latter region create temperature-dependent copy number phenotypes.
AB - Five different copA copy mutants of plasmid R1 have been identified by nucleotide sequencing. Independent measurements of the activities of the mutant inhibitor RNA and of the mutant target properties were carried out using several different methods. Correlation of these measurements with the location of th nucleotide substitutions resulted in the following conclusions: (1) The copy number of plasmid R1 is controlled primarily by interaction between the CopA RNA molecule and its target, the RepA mRNA. (2) The binding of th inhibitor to its target is based on nucleotide interactions within two complementary sequences of ten nucleotides and dependent on the secondary structure of the active site. (3) The secondary structure of both the CopA target and the CopA RNA is a stem-loop structure. Mutations in the loop region interfere with binding affinity between inhibitor and target, whereas mutations in the upper stem mainly interfere with secondary structure. Mutations in the latter region create temperature-dependent copy number phenotypes.
M3 - Journal article
C2 - 6203014
VL - 194
SP - 286
EP - 292
JO - Molecular General Genetics
JF - Molecular General Genetics
SN - 0026-8925
IS - 1-2
ER -
ID: 44293547