Congophilicity (Congo red affinity) of different beta2-microglobulin conformations characterized by dye affinity capillary electrophoresis.
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Congophilicity (Congo red affinity) of different beta2-microglobulin conformations characterized by dye affinity capillary electrophoresis. / Heegaard, N H; Sen, J W; Nissen, Mogens Holst.
In: Journal of Chromatography A, Vol. 894, No. 1-2, 2000, p. 319-27.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Congophilicity (Congo red affinity) of different beta2-microglobulin conformations characterized by dye affinity capillary electrophoresis.
AU - Heegaard, N H
AU - Sen, J W
AU - Nissen, Mogens Holst
N1 - Keywords: Coloring Agents; Congo Red; Electrophoresis, Capillary; Mass Spectrometry; Protein Conformation; beta 2-Microglobulin
PY - 2000
Y1 - 2000
N2 - The amyloidogenic protein beta-microglobulin was characterized by affinity capillary electrophoresis (CE). CE could separate conformational variants of beta2-microglobulin and with the amyloid-specific dye Congo red as a buffer additive it was possible to measure different Congo red-affinities of native and abnormally folded beta2-microglobulin. We find that native beta2-microglobulin has an intermediate affinity for Congo red at pH 7.3 and that binding involves electrostatic interactions. The conformational variant of beta2-microglobulin that appears in acetonitrile solutions binds Congo red more strongly. Affinity CE using Congo red as a buffer additive is a new, simple, fast, and quantitative micromethod for the characterization of soluble conformational intermediates of amyloidogenic proteins.
AB - The amyloidogenic protein beta-microglobulin was characterized by affinity capillary electrophoresis (CE). CE could separate conformational variants of beta2-microglobulin and with the amyloid-specific dye Congo red as a buffer additive it was possible to measure different Congo red-affinities of native and abnormally folded beta2-microglobulin. We find that native beta2-microglobulin has an intermediate affinity for Congo red at pH 7.3 and that binding involves electrostatic interactions. The conformational variant of beta2-microglobulin that appears in acetonitrile solutions binds Congo red more strongly. Affinity CE using Congo red as a buffer additive is a new, simple, fast, and quantitative micromethod for the characterization of soluble conformational intermediates of amyloidogenic proteins.
M3 - Journal article
C2 - 11100875
VL - 894
SP - 319
EP - 327
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0301-4770
IS - 1-2
ER -
ID: 8746353