Complete sequence of the extrachromosomal rDNA molecule from the ciliate Tetrahymena thermophila strain B1868VII
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Complete sequence of the extrachromosomal rDNA molecule from the ciliate Tetrahymena thermophila strain B1868VII. / Engberg, J; Nielsen, Henrik.
In: Nucleic Acids Research, Vol. 18, No. 23, 1990, p. 6915-9.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Complete sequence of the extrachromosomal rDNA molecule from the ciliate Tetrahymena thermophila strain B1868VII
AU - Engberg, J
AU - Nielsen, Henrik
N1 - Keywords: Animals; Base Sequence; DNA, Ribosomal; Extrachromosomal Inheritance; Molecular Sequence Data; Sequence Homology, Nucleic Acid; Tetrahymena; Transcription, Genetic
PY - 1990
Y1 - 1990
N2 - The recent development of rDNA vectors for transformation of Tetrahymena combined with improved microinjection technology should lead to a renewed interest in this organism. In particular, the rDNA itself constitutes an attractive system for biochemical studies. The rDNA is amplified to a level of 2% of the total DNA and exists as extrachromosomal molecules. Furthermore, the rDNA is homogeneous in sequence because it is derived from a single gene during sexual reorganization. In order to facilitate studies of this molecule, we report here a compilation of previously published sequence information together with new sequence data that completes the entire sequence of the 21 kb rDNA molecule.
AB - The recent development of rDNA vectors for transformation of Tetrahymena combined with improved microinjection technology should lead to a renewed interest in this organism. In particular, the rDNA itself constitutes an attractive system for biochemical studies. The rDNA is amplified to a level of 2% of the total DNA and exists as extrachromosomal molecules. Furthermore, the rDNA is homogeneous in sequence because it is derived from a single gene during sexual reorganization. In order to facilitate studies of this molecule, we report here a compilation of previously published sequence information together with new sequence data that completes the entire sequence of the 21 kb rDNA molecule.
M3 - Journal article
C2 - 2263454
VL - 18
SP - 6915
EP - 6919
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 23
ER -
ID: 14585689