Community-intrinsic properties enhance keratin degradation from bacterial consortia
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Community-intrinsic properties enhance keratin degradation from bacterial consortia. / Nasipuri, Poonam; Herschend, Jakob; Brejnrod, Asker D.; Madsen, Jonas S.; Espersen, Roall; Svensson, Birte; Burmølle, Mette; Jacquiod, Samuel; Sørensen, Søren J.
In: PLoS ONE, Vol. 15, No. 1, e0228108, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Community-intrinsic properties enhance keratin degradation from bacterial consortia
AU - Nasipuri, Poonam
AU - Herschend, Jakob
AU - Brejnrod, Asker D.
AU - Madsen, Jonas S.
AU - Espersen, Roall
AU - Svensson, Birte
AU - Burmølle, Mette
AU - Jacquiod, Samuel
AU - Sørensen, Søren J.
PY - 2020
Y1 - 2020
N2 - Although organic matter may accumulate sometimes (e.g. lignocellulose in peat bog), most natural biodegradation processes are completed until full mineralization. Such transformations are often achieved by the concerted action of communities of interacting microbes, involving different species each performing specific tasks. These interactions can give rise to novel “community-intrinsic” properties, through e.g. activation of so-called “silent genetic pathways” or synergistic interplay between microbial activities and functions. Here we studied the microbial community-based degradation of keratin, a recalcitrant biological material, by four soil isolates, which have previously been shown to display synergistic interactions during biofilm formation; Stenotrophomonas rhizophila, Xanthomonas retroflexus, Microbacterium oxydans and Paenibacillus amylolyticus. We observed enhanced keratin weight loss in cultures with X. retroflexus, both in dual and four-species co-cultures, as compared to expected keratin degradation by X. retroflexus alone. Additional community intrinsic properties included accelerated keratin degradation rates and increased biofilm formation on keratin particles. Comparison of secretome profiles of X. retroflexus mono-cultures to cocultures revealed that certain proteases (e.g. serine protease S08) were significantly more abundant in mono-cultures, whereas co-cultures had an increased abundance of proteins related to maintaining the redox environment, e.g. glutathione peroxidase. Hence, one of the mechanisms related to the community intrinsic properties, leading to enhanced degradation from co-cultures, might be related to a switch from sulfitolytic to proteolytic functions between mono- and co-cultures, respectively.
AB - Although organic matter may accumulate sometimes (e.g. lignocellulose in peat bog), most natural biodegradation processes are completed until full mineralization. Such transformations are often achieved by the concerted action of communities of interacting microbes, involving different species each performing specific tasks. These interactions can give rise to novel “community-intrinsic” properties, through e.g. activation of so-called “silent genetic pathways” or synergistic interplay between microbial activities and functions. Here we studied the microbial community-based degradation of keratin, a recalcitrant biological material, by four soil isolates, which have previously been shown to display synergistic interactions during biofilm formation; Stenotrophomonas rhizophila, Xanthomonas retroflexus, Microbacterium oxydans and Paenibacillus amylolyticus. We observed enhanced keratin weight loss in cultures with X. retroflexus, both in dual and four-species co-cultures, as compared to expected keratin degradation by X. retroflexus alone. Additional community intrinsic properties included accelerated keratin degradation rates and increased biofilm formation on keratin particles. Comparison of secretome profiles of X. retroflexus mono-cultures to cocultures revealed that certain proteases (e.g. serine protease S08) were significantly more abundant in mono-cultures, whereas co-cultures had an increased abundance of proteins related to maintaining the redox environment, e.g. glutathione peroxidase. Hence, one of the mechanisms related to the community intrinsic properties, leading to enhanced degradation from co-cultures, might be related to a switch from sulfitolytic to proteolytic functions between mono- and co-cultures, respectively.
U2 - 10.1371/journal.pone.0228108
DO - 10.1371/journal.pone.0228108
M3 - Journal article
C2 - 32004342
AN - SCOPUS:85078716759
VL - 15
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 1
M1 - e0228108
ER -
ID: 236713626