Circulating but not faecal short-chain fatty acids are related to insulin sensitivity, lipolysis and GLP-1 concentrations in humans
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Circulating but not faecal short-chain fatty acids are related to insulin sensitivity, lipolysis and GLP-1 concentrations in humans. / Muller, Mattea; Hernandez, Manuel A. Gonzalez; Goossens, Gijs H.; Reijnders, Dorien; Holst, Jens J.; Jocken, Johan W. E.; van Eijk, Hans; Canfora, Emanuel E.; Blaak, Ellen E.
In: Scientific Reports, Vol. 9, 12515, 2019.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Circulating but not faecal short-chain fatty acids are related to insulin sensitivity, lipolysis and GLP-1 concentrations in humans
AU - Muller, Mattea
AU - Hernandez, Manuel A. Gonzalez
AU - Goossens, Gijs H.
AU - Reijnders, Dorien
AU - Holst, Jens J.
AU - Jocken, Johan W. E.
AU - van Eijk, Hans
AU - Canfora, Emanuel E.
AU - Blaak, Ellen E.
PY - 2019
Y1 - 2019
N2 - Microbial-derived short-chain fatty acids (SCFA) acetate, propionate and butyrate may provide a link between gut microbiota and whole-body insulin sensitivity (IS). In this cross-sectional study (160 participants, 64% male, BMI: 19.2-41.0kg/m(2), normal or impaired glucose metabolism), associations between SCFA (faecal and fasting circulating) and circulating metabolites, substrate oxidation and IS were investigated. In a subgroup (n = 93), IS was determined using a hyperinsulinemic-euglycemic clamp. Data were analyzed using multiple linear regression analysis adjusted for sex, age and BMI. Fasting circulating acetate, propionate and butyrate concentrations were positively associated with fasting GLP-1 concentrations. Additionally, circulating SCFA were negatively related to whole-body lipolysis (glycerol), triacylglycerols and free fatty acids levels (standardized (std) beta adjusted (adj) - 0.190, P = 0.023; std beta adj - 0.202, P = 0.010; std beta adj - 0.306, P = 0.001, respectively). Circulating acetate and propionate were, respectively, negatively and positively correlated with IS (M-value: std beta adj - 0.294, P < 0.001; std beta adj 0.161, P = 0.033, respectively). We show that circulating rather than faecal SCFA were associated with GLP-1 concentrations, whole-body lipolysis and peripheral IS in humans. Therefore, circulating SCFA are more directly linked to metabolic health, which indicates the need to measure circulating SCFA in human prebiotic/probiotic intervention studies as a biomarker/mediator of effects on host metabolism.
AB - Microbial-derived short-chain fatty acids (SCFA) acetate, propionate and butyrate may provide a link between gut microbiota and whole-body insulin sensitivity (IS). In this cross-sectional study (160 participants, 64% male, BMI: 19.2-41.0kg/m(2), normal or impaired glucose metabolism), associations between SCFA (faecal and fasting circulating) and circulating metabolites, substrate oxidation and IS were investigated. In a subgroup (n = 93), IS was determined using a hyperinsulinemic-euglycemic clamp. Data were analyzed using multiple linear regression analysis adjusted for sex, age and BMI. Fasting circulating acetate, propionate and butyrate concentrations were positively associated with fasting GLP-1 concentrations. Additionally, circulating SCFA were negatively related to whole-body lipolysis (glycerol), triacylglycerols and free fatty acids levels (standardized (std) beta adjusted (adj) - 0.190, P = 0.023; std beta adj - 0.202, P = 0.010; std beta adj - 0.306, P = 0.001, respectively). Circulating acetate and propionate were, respectively, negatively and positively correlated with IS (M-value: std beta adj - 0.294, P < 0.001; std beta adj 0.161, P = 0.033, respectively). We show that circulating rather than faecal SCFA were associated with GLP-1 concentrations, whole-body lipolysis and peripheral IS in humans. Therefore, circulating SCFA are more directly linked to metabolic health, which indicates the need to measure circulating SCFA in human prebiotic/probiotic intervention studies as a biomarker/mediator of effects on host metabolism.
U2 - 10.1038/s41598-019-48775-0
DO - 10.1038/s41598-019-48775-0
M3 - Journal article
C2 - 31467327
VL - 9
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 12515
ER -
ID: 227566959