Cell culture system of a hepatitis C genotype 3a and 2a chimera: Patent #: 8,945,584
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Cell culture system of a hepatitis C genotype 3a and 2a chimera : Patent #: 8,945,584. / Gottwein, Judith Margarete (Inventor); Scheel, Troels Kasper Hoyer (Inventor); Eugen-Olsen, Jesper (Inventor); Bukh, Jens (Inventor).
Patent No.: WO2008/125117. Feb 03, 2015.Research output: Patent
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TY - PAT
T1 - Cell culture system of a hepatitis C genotype 3a and 2a chimera
T2 - Patent #: 8,945,584
AU - Gottwein, Judith Margarete
AU - Scheel, Troels Kasper Hoyer
AU - Eugen-Olsen, Jesper
AU - Bukh, Jens
PY - 2015/2/3
Y1 - 2015/2/3
N2 - A robust and genetically stable cell culture system for Hepatitis C Virus (HCV) genotype 3a is provided. A genotype 3a/2a (S52/JFH1) recombinant containing the structural genes (Core, E1, E2), p7 and NS2 of strain S52 was constructed and characterized in Huh7.5 cells. S52/JFH1 and J6/JFH viruses passaged in cell culture had comparable growth kinetics and yielded similar peak HCV RNA titers and infectivity titers. Direct genome sequencing of cell culture derived S52/JFH1 viruses identified putative adaptive mutations in Core, E2, p7, NS3, and NS5A; clonal analysis revealed that all genomes analyzed exhibited different combinations of these mutations. Finally, viruses resulting from transfection with RNA transcripts of five S52/JFH1 recombinants containing these combinations of putative adaptive mutations performed as efficiently as J6/JFH viruses in Huh7.5 cells and were all genetically stable after viral passage.
AB - A robust and genetically stable cell culture system for Hepatitis C Virus (HCV) genotype 3a is provided. A genotype 3a/2a (S52/JFH1) recombinant containing the structural genes (Core, E1, E2), p7 and NS2 of strain S52 was constructed and characterized in Huh7.5 cells. S52/JFH1 and J6/JFH viruses passaged in cell culture had comparable growth kinetics and yielded similar peak HCV RNA titers and infectivity titers. Direct genome sequencing of cell culture derived S52/JFH1 viruses identified putative adaptive mutations in Core, E2, p7, NS3, and NS5A; clonal analysis revealed that all genomes analyzed exhibited different combinations of these mutations. Finally, viruses resulting from transfection with RNA transcripts of five S52/JFH1 recombinants containing these combinations of putative adaptive mutations performed as efficiently as J6/JFH viruses in Huh7.5 cells and were all genetically stable after viral passage.
M3 - Patent
M1 - WO2008/125117
Y2 - 2008/10/23
ER -
ID: 140391544