Cell culture system of a hepatitis C genotype 3a and 2a chimera: Patent #: 8,945,584

Research output: Patent

Standard

Cell culture system of a hepatitis C genotype 3a and 2a chimera : Patent #: 8,945,584. / Gottwein, Judith Margarete (Inventor); Scheel, Troels Kasper Hoyer (Inventor); Eugen-Olsen, Jesper (Inventor); Bukh, Jens (Inventor).

Patent No.: WO2008/125117. Feb 03, 2015.

Research output: Patent

Harvard

Gottwein, JM, Scheel, TKH, Eugen-Olsen, J & Bukh, J Feb. 03 2015, Cell culture system of a hepatitis C genotype 3a and 2a chimera: Patent #: 8,945,584, Patent No. WO2008/125117.

APA

Gottwein, J. M., Scheel, T. K. H., Eugen-Olsen, J., & Bukh, J. (2015). Cell culture system of a hepatitis C genotype 3a and 2a chimera: Patent #: 8,945,584. (Patent No. WO2008/125117).

Vancouver

Gottwein JM, Scheel TKH, Eugen-Olsen J, Bukh J, inventors. Cell culture system of a hepatitis C genotype 3a and 2a chimera: Patent #: 8,945,584. WO2008/125117. 2015 Feb 3.

Author

Gottwein, Judith Margarete (Inventor) ; Scheel, Troels Kasper Hoyer (Inventor) ; Eugen-Olsen, Jesper (Inventor) ; Bukh, Jens (Inventor). / Cell culture system of a hepatitis C genotype 3a and 2a chimera : Patent #: 8,945,584. Patent No.: WO2008/125117. Feb 03, 2015.

Bibtex

@misc{0e5a0d1af40c4b6dab73d2da4a33ced4,
title = "Cell culture system of a hepatitis C genotype 3a and 2a chimera: Patent #: 8,945,584",
abstract = "A robust and genetically stable cell culture system for Hepatitis C Virus (HCV) genotype 3a is provided. A genotype 3a/2a (S52/JFH1) recombinant containing the structural genes (Core, E1, E2), p7 and NS2 of strain S52 was constructed and characterized in Huh7.5 cells. S52/JFH1 and J6/JFH viruses passaged in cell culture had comparable growth kinetics and yielded similar peak HCV RNA titers and infectivity titers. Direct genome sequencing of cell culture derived S52/JFH1 viruses identified putative adaptive mutations in Core, E2, p7, NS3, and NS5A; clonal analysis revealed that all genomes analyzed exhibited different combinations of these mutations. Finally, viruses resulting from transfection with RNA transcripts of five S52/JFH1 recombinants containing these combinations of putative adaptive mutations performed as efficiently as J6/JFH viruses in Huh7.5 cells and were all genetically stable after viral passage.",
author = "Gottwein, {Judith Margarete} and Scheel, {Troels Kasper Hoyer} and Jesper Eugen-Olsen and Jens Bukh",
year = "2015",
month = feb,
day = "3",
language = "English",
type = "Patent",
note = "WO2008/125117; A61K 39/29 (20060101); C12N 15/06 (20060101); C12Q 1/02 (20060101); C07H 21/00 (20060101); C12N 7/02 (20060101); A61K 39/12 (20060101)",

}

RIS

TY - PAT

T1 - Cell culture system of a hepatitis C genotype 3a and 2a chimera

T2 - Patent #: 8,945,584

AU - Gottwein, Judith Margarete

AU - Scheel, Troels Kasper Hoyer

AU - Eugen-Olsen, Jesper

AU - Bukh, Jens

PY - 2015/2/3

Y1 - 2015/2/3

N2 - A robust and genetically stable cell culture system for Hepatitis C Virus (HCV) genotype 3a is provided. A genotype 3a/2a (S52/JFH1) recombinant containing the structural genes (Core, E1, E2), p7 and NS2 of strain S52 was constructed and characterized in Huh7.5 cells. S52/JFH1 and J6/JFH viruses passaged in cell culture had comparable growth kinetics and yielded similar peak HCV RNA titers and infectivity titers. Direct genome sequencing of cell culture derived S52/JFH1 viruses identified putative adaptive mutations in Core, E2, p7, NS3, and NS5A; clonal analysis revealed that all genomes analyzed exhibited different combinations of these mutations. Finally, viruses resulting from transfection with RNA transcripts of five S52/JFH1 recombinants containing these combinations of putative adaptive mutations performed as efficiently as J6/JFH viruses in Huh7.5 cells and were all genetically stable after viral passage.

AB - A robust and genetically stable cell culture system for Hepatitis C Virus (HCV) genotype 3a is provided. A genotype 3a/2a (S52/JFH1) recombinant containing the structural genes (Core, E1, E2), p7 and NS2 of strain S52 was constructed and characterized in Huh7.5 cells. S52/JFH1 and J6/JFH viruses passaged in cell culture had comparable growth kinetics and yielded similar peak HCV RNA titers and infectivity titers. Direct genome sequencing of cell culture derived S52/JFH1 viruses identified putative adaptive mutations in Core, E2, p7, NS3, and NS5A; clonal analysis revealed that all genomes analyzed exhibited different combinations of these mutations. Finally, viruses resulting from transfection with RNA transcripts of five S52/JFH1 recombinants containing these combinations of putative adaptive mutations performed as efficiently as J6/JFH viruses in Huh7.5 cells and were all genetically stable after viral passage.

M3 - Patent

M1 - WO2008/125117

Y2 - 2008/10/23

ER -

ID: 140391544