Calcium transport in male reproduction is possibly influenced by vitamin D and CaSR
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Calcium transport in male reproduction is possibly influenced by vitamin D and CaSR. / Boisen, Ida Marie; Nielsen, John Erik; Verlinden, Lieve; Lorenzen, Mette; Holt, Rune; Pinborg, Anja; Andreassen, Christine Hjorth; Juul, Anders; Lanske, Beate; Carmeliet, Geert; Blomberg Jensen, Martin.
In: The Journal of endocrinology, Vol. 251, No. 3, 2021, p. 207-222.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Calcium transport in male reproduction is possibly influenced by vitamin D and CaSR
AU - Boisen, Ida Marie
AU - Nielsen, John Erik
AU - Verlinden, Lieve
AU - Lorenzen, Mette
AU - Holt, Rune
AU - Pinborg, Anja
AU - Andreassen, Christine Hjorth
AU - Juul, Anders
AU - Lanske, Beate
AU - Carmeliet, Geert
AU - Blomberg Jensen, Martin
PY - 2021
Y1 - 2021
N2 - Vitamin D is important for gonadal function in rodents, and improvement of vitamin D status in men with low sperm counts increases live birth rate. Vitamin D is a regulator of transcellular calcium transport in the intestine and kidney and may influence the dramatic changes in the luminal calcium concentration in epididymis. Here, we show spatial expression in the male reproductive tract of vitamin D receptor (VDR)-regulated factors involved in calcium transport: transient receptor potential vanilloid 5/6 , sodium/calcium exchanger 1, plasma membrane calcium ATPase 1, calbindin D9k, calcium-sensing receptor (CaSR), and parathyroid hormone-related peptide (PTHrP) in mouse and human testis and epididymis. Testicular Casr expression was lower in Vdr ablated mice compared with controls. Moreover, expression levels of Casr and Pthrp were strongly correlated in both testis and epididymis and Pthrp was suppressed by 1,25(OH)2D3 in a spermatogonial cell line. The expression of CaSR in epididymis may be of greater importance than in the gonad in mice as germ cell-specific Casr deficient mice had no major reproductive phenotype, and coincubation with a CaSR-agonist had no effect on human sperm-oocyte binding. In humans, seminal calcium concentration between 5 and 10 mM was associated with a higher fraction of motile and morphologically normal sperm cells, and the seminal calcium concentration was not associated with serum calcium levels. In conclusion, VDR regulates CaSR and PTHrP, and both factors may be involved in the regulation of calcium transport in the male reproductive tract with possible implications for sperm function and storage.
AB - Vitamin D is important for gonadal function in rodents, and improvement of vitamin D status in men with low sperm counts increases live birth rate. Vitamin D is a regulator of transcellular calcium transport in the intestine and kidney and may influence the dramatic changes in the luminal calcium concentration in epididymis. Here, we show spatial expression in the male reproductive tract of vitamin D receptor (VDR)-regulated factors involved in calcium transport: transient receptor potential vanilloid 5/6 , sodium/calcium exchanger 1, plasma membrane calcium ATPase 1, calbindin D9k, calcium-sensing receptor (CaSR), and parathyroid hormone-related peptide (PTHrP) in mouse and human testis and epididymis. Testicular Casr expression was lower in Vdr ablated mice compared with controls. Moreover, expression levels of Casr and Pthrp were strongly correlated in both testis and epididymis and Pthrp was suppressed by 1,25(OH)2D3 in a spermatogonial cell line. The expression of CaSR in epididymis may be of greater importance than in the gonad in mice as germ cell-specific Casr deficient mice had no major reproductive phenotype, and coincubation with a CaSR-agonist had no effect on human sperm-oocyte binding. In humans, seminal calcium concentration between 5 and 10 mM was associated with a higher fraction of motile and morphologically normal sperm cells, and the seminal calcium concentration was not associated with serum calcium levels. In conclusion, VDR regulates CaSR and PTHrP, and both factors may be involved in the regulation of calcium transport in the male reproductive tract with possible implications for sperm function and storage.
KW - calcium
KW - CaSR
KW - fertility
KW - reproduction
KW - testis
KW - vitamin D
U2 - 10.1530/JOE-20-0321
DO - 10.1530/JOE-20-0321
M3 - Journal article
C2 - 34612843
AN - SCOPUS:85121957828
VL - 251
SP - 207
EP - 222
JO - Journal of Endocrinology
JF - Journal of Endocrinology
SN - 0022-0795
IS - 3
ER -
ID: 290447957