Beta-cell lines derived from transgenic mice expressing a hybrid insulin gene-oncogene
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Beta-cell lines derived from transgenic mice expressing a hybrid insulin gene-oncogene. / Efrat, S; Linde, S; Kofod, Hans; Spector, D; Delannoy, M; Grant, S; Hanahan, D; Baekkeskov, S.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 23, 12.1988, p. 9037-41.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Beta-cell lines derived from transgenic mice expressing a hybrid insulin gene-oncogene
AU - Efrat, S
AU - Linde, S
AU - Kofod, Hans
AU - Spector, D
AU - Delannoy, M
AU - Grant, S
AU - Hanahan, D
AU - Baekkeskov, S
PY - 1988/12
Y1 - 1988/12
N2 - Three pancreatic beta-cell lines have been established from insulinomas derived from transgenic mice carrying a hybrid insulin-promoted simian virus 40 tumor antigen gene. The beta tumor cell (beta TC) lines maintain the features of differentiated beta cells for about 50 passages in culture. The cells produce both proinsulin I and II and efficiently process each into mature insulin, in a manner comparable to normal beta cells in isolated islets. Electron microscopy reveals typical beta-cell type secretory granules, in which insulin is stored. Insulin secretion is inducible up to 30-fold by glucose, although with a lower threshold for maximal stimulation than that for normal beta cells. beta TC lines can be repeatedly derived from primary beta-cell tumors that heritably arise in the transgenic mice. Thus, targeted expression of an oncogene with a cell-specific regulatory element can be used both to immortalize a rare cell type and to provide a selection for the maintenance of its differentiated phenotype.
AB - Three pancreatic beta-cell lines have been established from insulinomas derived from transgenic mice carrying a hybrid insulin-promoted simian virus 40 tumor antigen gene. The beta tumor cell (beta TC) lines maintain the features of differentiated beta cells for about 50 passages in culture. The cells produce both proinsulin I and II and efficiently process each into mature insulin, in a manner comparable to normal beta cells in isolated islets. Electron microscopy reveals typical beta-cell type secretory granules, in which insulin is stored. Insulin secretion is inducible up to 30-fold by glucose, although with a lower threshold for maximal stimulation than that for normal beta cells. beta TC lines can be repeatedly derived from primary beta-cell tumors that heritably arise in the transgenic mice. Thus, targeted expression of an oncogene with a cell-specific regulatory element can be used both to immortalize a rare cell type and to provide a selection for the maintenance of its differentiated phenotype.
KW - Adenoma, Islet Cell
KW - Animals
KW - Antigens, Viral, Tumor
KW - Genes
KW - Insulin
KW - Insulinoma
KW - Islets of Langerhans
KW - Mice
KW - Mice, Transgenic
KW - Oncogenes
KW - Pancreatic Neoplasms
KW - Simian virus 40
KW - Tumor Cells, Cultured
M3 - Journal article
C2 - 2848253
VL - 85
SP - 9037
EP - 9041
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 23
ER -
ID: 45575125