Associations between common intestinal parasites and bacteria in humans as revealed by qPCR
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Associations between common intestinal parasites and bacteria in humans as revealed by qPCR. / O'Brien Andersen, L.; Karim, A. B.; Roager, Henrik Munch; Vigsnæs, L.K.; Krogfelt, K.A.; Licht, T.R.; Stensvold, C. R.
In: European Journal of Clinical Microbiology & Infectious Diseases, Vol. 35, No. 9, 2016, p. 1427-1431.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Associations between common intestinal parasites and bacteria in humans as revealed by qPCR
AU - O'Brien Andersen, L.
AU - Karim, A. B.
AU - Roager, Henrik Munch
AU - Vigsnæs, L.K.
AU - Krogfelt, K.A.
AU - Licht, T.R.
AU - Stensvold, C. R.
PY - 2016
Y1 - 2016
N2 - Several studies have shown associations between groups of intestinal bacterial or specific ratios between bacterial groups and various disease traits. Meanwhile, little is known about interactions and associations between eukaryotic and prokaryotic microorganisms in the human gut. In this work, we set out to investigate potential associations between common single-celled parasites such as Blastocystis spp. and Dientamoeba fragilis and intestinal bacteria. Stool DNA from patients with intestinal symptoms were selected based on being Blastocystis spp.-positive (B+)/negative (B-) and D. fragilis-positive (D+)/negative (D-), and split into four groups of 21 samples (B+ D+, B+ D-, B- D+, and B- D-). Quantitative PCR targeting the six bacterial taxa Bacteroides, Prevotella, the butyrate-producing clostridial clusters IV and XIVa, the mucin-degrading Akkermansia muciniphila, and the indigenous group of Bifidobacterium was subsequently performed, and the relative abundance of these bacteria across the four groups was compared. The relative abundance of Bacteroides in B- D- samples was significantly higher compared with B+ D- and B+ D+ samples (P
AB - Several studies have shown associations between groups of intestinal bacterial or specific ratios between bacterial groups and various disease traits. Meanwhile, little is known about interactions and associations between eukaryotic and prokaryotic microorganisms in the human gut. In this work, we set out to investigate potential associations between common single-celled parasites such as Blastocystis spp. and Dientamoeba fragilis and intestinal bacteria. Stool DNA from patients with intestinal symptoms were selected based on being Blastocystis spp.-positive (B+)/negative (B-) and D. fragilis-positive (D+)/negative (D-), and split into four groups of 21 samples (B+ D+, B+ D-, B- D+, and B- D-). Quantitative PCR targeting the six bacterial taxa Bacteroides, Prevotella, the butyrate-producing clostridial clusters IV and XIVa, the mucin-degrading Akkermansia muciniphila, and the indigenous group of Bifidobacterium was subsequently performed, and the relative abundance of these bacteria across the four groups was compared. The relative abundance of Bacteroides in B- D- samples was significantly higher compared with B+ D- and B+ D+ samples (P
KW - Biomedicine, Medical Microbiology, Internal Medicine, SC3
U2 - 10.1007/s10096-016-2680-2
DO - 10.1007/s10096-016-2680-2
M3 - Journal article
VL - 35
SP - 1427
EP - 1431
JO - European Journal of Clinical Microbiology & Infectious Diseases
JF - European Journal of Clinical Microbiology & Infectious Diseases
SN - 0934-9723
IS - 9
ER -
ID: 191895704