Analysis of human faecal host proteins: Responsiveness to 10-week dietary intervention modifying dietary protein intake in elderly males
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Analysis of human faecal host proteins: Responsiveness to 10-week dietary intervention modifying dietary protein intake in elderly males. / Gathercole, Jessica L; Grosvenor, Anita J; Lee, Erin; Thomas, Ancy; Mitchell, Cameron J; Zeng, Nina; D'Souza, Randall F; Ramzan, Farha; Sharma, Pankaja; Knowles, Scott O; Roy, Nicole C; Sjödin, Anders; Wagner, Karl-Heinz; Milan, Amber M; Mitchell, Sarah M; Cameron-Smith, David.
In: Frontiers in Nutrition, Vol. 7, 595905, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Analysis of human faecal host proteins: Responsiveness to 10-week dietary intervention modifying dietary protein intake in elderly males
AU - Gathercole, Jessica L
AU - Grosvenor, Anita J
AU - Lee, Erin
AU - Thomas, Ancy
AU - Mitchell, Cameron J
AU - Zeng, Nina
AU - D'Souza, Randall F
AU - Ramzan, Farha
AU - Sharma, Pankaja
AU - Knowles, Scott O
AU - Roy, Nicole C
AU - Sjödin, Anders
AU - Wagner, Karl-Heinz
AU - Milan, Amber M
AU - Mitchell, Sarah M
AU - Cameron-Smith, David
N1 - CURIS 2021 NEXS 062
PY - 2021
Y1 - 2021
N2 - Faecal proteomics targeting biomarkers of immunity and inflammation have demonstrated clinical application for the identification of changes in gastrointestinal function. However, there are limited comprehensive analyses of the host faecal proteome and how it may be influenced by dietary factors. To examine this, the Homo sapiens post-diet proteome of older males was analysed at the completion of a 10-week dietary intervention, either meeting the minimum dietary protein recommendations (RDA; n = 9) or twice the recommended dietary allowance (2RDA, n = 10). The host faecal proteome differed markedly between individuals, with only a small subset of proteins present in ≥ 60% of subjects (14 and 44 proteins, RDA and 2RDA, respectively, with only 7 common to both groups). No differences were observed between the diet groups on the profiles of host faecal proteins. Faecal proteins were detected from a wide range of protein classes, with high inter-individual variation and absence of obvious impact in response to diets with markedly different protein intake. This suggests that well-matched whole food diets with two-fold variation in protein intake maintained for 10 weeks have minimal impact on human faecal host proteins.
AB - Faecal proteomics targeting biomarkers of immunity and inflammation have demonstrated clinical application for the identification of changes in gastrointestinal function. However, there are limited comprehensive analyses of the host faecal proteome and how it may be influenced by dietary factors. To examine this, the Homo sapiens post-diet proteome of older males was analysed at the completion of a 10-week dietary intervention, either meeting the minimum dietary protein recommendations (RDA; n = 9) or twice the recommended dietary allowance (2RDA, n = 10). The host faecal proteome differed markedly between individuals, with only a small subset of proteins present in ≥ 60% of subjects (14 and 44 proteins, RDA and 2RDA, respectively, with only 7 common to both groups). No differences were observed between the diet groups on the profiles of host faecal proteins. Faecal proteins were detected from a wide range of protein classes, with high inter-individual variation and absence of obvious impact in response to diets with markedly different protein intake. This suggests that well-matched whole food diets with two-fold variation in protein intake maintained for 10 weeks have minimal impact on human faecal host proteins.
KW - Dietary protein
KW - Faeces
KW - Gastrointestinal health
KW - Host proteins
KW - Proteomics
UR - http://www.scopus.com/inward/record.url?scp=85100024454&partnerID=8YFLogxK
U2 - 10.3389/fnut.2020.595905
DO - 10.3389/fnut.2020.595905
M3 - Journal article
C2 - 33521034
AN - SCOPUS:85100024454
VL - 7
JO - Frontiers in Nutrition
JF - Frontiers in Nutrition
SN - 2296-861X
M1 - 595905
ER -
ID: 256577727