Adipose tissue monomethyl branched-chain fatty acids and insulin sensitivity: Effects of obesity and weight loss
Research output: Contribution to journal › Journal article › Research › peer-review
Objectives: An increase in circulating branched-chain amino acids (BCAA) is associated with insulin resistance. Adipose tissue is a potentially important site for BCAA metabolism. It was evaluated whether monomethyl branched-chain fatty acids (mmBCFA) in adipose tissue, which are likely derived from BCAA catabolism, are associated with insulin sensitivity.
Methods: Insulin-stimulated glucose disposal was determined by using the hyperinsulinemic-euglycemic clamp procedure with stable isotope glucose tracer infusion in nine lean and nine obese subjects, and in a separate group of nine obese subjects before and 1 year after Roux-en-Y gastric bypass (RYGB) surgery (38% weight loss). Adipose tissue mmBCFA content was measured in tissue biopsies taken in the basal state.
Results: Total adipose tissue mmBCFA content was ∼30% lower in obese than lean subjects (P = 0.02) and increased by ∼65% after weight loss in the RYGB group (P = 0.01). Adipose tissue mmBCFA content correlated positively with skeletal muscle insulin sensitivity (R2 =35%, P = 0.01, n = 18).
Conclusions: These results demonstrate a novel association between adipose tissue mmBCFA content and obesity-related insulin resistance. Additional studies are needed to determine whether the association between adipose tissue mmBCFA and muscle insulin sensitivity is causal or a simple association.
Original language | English |
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Journal | Obesity |
Volume | 23 |
Issue number | 2 |
Pages (from-to) | 329-334 |
Number of pages | 6 |
ISSN | 1930-7381 |
DOIs | |
Publication status | Published - 2015 |
Externally published | Yes |
Bibliographical note
© 2014 The Obesity Society.
- Adipose Tissue/metabolism, Adult, Cross-Sectional Studies, Fatty Acids/metabolism, Female, Gastric Bypass, Glucose Clamp Technique, Humans, Insulin/metabolism, Insulin Resistance/physiology, Longitudinal Studies, Male, Middle Aged, Obesity/metabolism, Weight Loss/physiology
Research areas
ID: 289963282