Accessible high-speed image-activated cell sorting
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Accessible high-speed image-activated cell sorting. / Kuhn, Terra M.; Paulsen, Malte; Cuylen-Haering, Sara.
In: Trends in Cell Biology, 2024.Research output: Contribution to journal › Review › Research › peer-review
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TY - JOUR
T1 - Accessible high-speed image-activated cell sorting
AU - Kuhn, Terra M.
AU - Paulsen, Malte
AU - Cuylen-Haering, Sara
N1 - Publisher Copyright: © 2024 The Authors
PY - 2024
Y1 - 2024
N2 - Over the past six decades, fluorescence-activated cell sorting (FACS) has become an essential technology for basic and clinical research by enabling the isolation of cells of interest in high throughput. Recent technological advancements have started a new era of flow cytometry. By combining the spatial resolution of microscopy with high-speed cell sorting, new instruments allow cell sorting based on simple image-derived parameters or sophisticated image analysis algorithms, thereby greatly expanding the scope of applications. In this review, we discuss the systems that are commercially available or have been described in enough methodological and engineering detail to allow their replication. We summarize their strengths and limitations and highlight applications that have the potential to transform various fields in basic life science research and clinical settings.
AB - Over the past six decades, fluorescence-activated cell sorting (FACS) has become an essential technology for basic and clinical research by enabling the isolation of cells of interest in high throughput. Recent technological advancements have started a new era of flow cytometry. By combining the spatial resolution of microscopy with high-speed cell sorting, new instruments allow cell sorting based on simple image-derived parameters or sophisticated image analysis algorithms, thereby greatly expanding the scope of applications. In this review, we discuss the systems that are commercially available or have been described in enough methodological and engineering detail to allow their replication. We summarize their strengths and limitations and highlight applications that have the potential to transform various fields in basic life science research and clinical settings.
KW - cell sorting
KW - flow cytometry
KW - high-throughput imaging
KW - image analysis
U2 - 10.1016/j.tcb.2024.04.007
DO - 10.1016/j.tcb.2024.04.007
M3 - Review
C2 - 38789300
AN - SCOPUS:85194032146
JO - Trends in Cell Biology
JF - Trends in Cell Biology
SN - 0962-8924
ER -
ID: 394477016