The fibroblast growth factor receptor (FGFR) agonist FGF1 and the neural cell adhesion molecule-derived peptide FGL activate FGFR substrate 2alpha differently
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The fibroblast growth factor receptor (FGFR) agonist FGF1 and the neural cell adhesion molecule-derived peptide FGL activate FGFR substrate 2alpha differently. / Chen, Yongshuo; Li, Shizhong; Berezin, Vladimir; Bock, Elisabeth.
I: Journal of Neuroscience Research, Bind 88, Nr. 9, 01.07.2010, s. 1882-9.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - The fibroblast growth factor receptor (FGFR) agonist FGF1 and the neural cell adhesion molecule-derived peptide FGL activate FGFR substrate 2alpha differently
AU - Chen, Yongshuo
AU - Li, Shizhong
AU - Berezin, Vladimir
AU - Bock, Elisabeth
PY - 2010/7/1
Y1 - 2010/7/1
N2 - Activation of fibroblast growth factor (FGF) receptors (FGFRs) both by FGFs and by the neural cell adhesion molecule (NCAM) is crucial in the development and function of the nervous system. We found that FGFR substrate 2alpha (FRS2alpha), Src homologous and collagen A (ShcA), and phospholipase-Cgamma (PLCgamma) were all required for neurite outgrowth from cerebellar granule neurons (CGNs) induced by FGF1 and FGL (an NCAM-derived peptide agonist of FGFR1). Like FGF1, FGL induced tyrosine phosphorylation of FGFR1, FRS2alpha, ShcA, and PLCgamma in a time- and dose-dependent manner. However, the activation of FRS2alpha by FGL was significantly lower than the activation by FGF1, indicating a differential signaling profile induced by NCAM compared with the cognate growth factor.
AB - Activation of fibroblast growth factor (FGF) receptors (FGFRs) both by FGFs and by the neural cell adhesion molecule (NCAM) is crucial in the development and function of the nervous system. We found that FGFR substrate 2alpha (FRS2alpha), Src homologous and collagen A (ShcA), and phospholipase-Cgamma (PLCgamma) were all required for neurite outgrowth from cerebellar granule neurons (CGNs) induced by FGF1 and FGL (an NCAM-derived peptide agonist of FGFR1). Like FGF1, FGL induced tyrosine phosphorylation of FGFR1, FRS2alpha, ShcA, and PLCgamma in a time- and dose-dependent manner. However, the activation of FRS2alpha by FGL was significantly lower than the activation by FGF1, indicating a differential signaling profile induced by NCAM compared with the cognate growth factor.
KW - Adaptor Proteins, Signal Transducing
KW - Animals
KW - Cell Enlargement
KW - Cells, Cultured
KW - Cerebellum
KW - Fibroblast Growth Factor 1
KW - Models, Neurological
KW - Neural Cell Adhesion Molecules
KW - Neurites
KW - Neurons
KW - Phospholipase C gamma
KW - Phosphorylation
KW - Rats
KW - Rats, Wistar
KW - Receptors, Fibroblast Growth Factor
KW - Shc Signaling Adaptor Proteins
KW - Time Factors
KW - Tyrosine
U2 - 10.1002/jnr.22374
DO - 10.1002/jnr.22374
M3 - Journal article
C2 - 20175207
VL - 88
SP - 1882
EP - 1889
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
SN - 0360-4012
IS - 9
ER -
ID: 35293015