Enzyme-linked immunosorbent assay for activation of the classical complement pathway by Plasmodium falciparum-infected erythrocyte surface antigen-specific antibodies

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Standard

Enzyme-linked immunosorbent assay for activation of the classical complement pathway by Plasmodium falciparum-infected erythrocyte surface antigen-specific antibodies. / Larsen, Mads Delbo; Bayarri-Olmos, Rafael; Garred, Peter; Hviid, Lars.

I: Methods in Molecular Biology, Bind 2470, 2022, s. 673-678.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Larsen, MD, Bayarri-Olmos, R, Garred, P & Hviid, L 2022, 'Enzyme-linked immunosorbent assay for activation of the classical complement pathway by Plasmodium falciparum-infected erythrocyte surface antigen-specific antibodies', Methods in Molecular Biology, bind 2470, s. 673-678. https://doi.org/10.1007/978-1-0716-2189-9_50

APA

Larsen, M. D., Bayarri-Olmos, R., Garred, P., & Hviid, L. (2022). Enzyme-linked immunosorbent assay for activation of the classical complement pathway by Plasmodium falciparum-infected erythrocyte surface antigen-specific antibodies. Methods in Molecular Biology, 2470, 673-678. https://doi.org/10.1007/978-1-0716-2189-9_50

Vancouver

Larsen MD, Bayarri-Olmos R, Garred P, Hviid L. Enzyme-linked immunosorbent assay for activation of the classical complement pathway by Plasmodium falciparum-infected erythrocyte surface antigen-specific antibodies. Methods in Molecular Biology. 2022;2470:673-678. https://doi.org/10.1007/978-1-0716-2189-9_50

Author

Larsen, Mads Delbo ; Bayarri-Olmos, Rafael ; Garred, Peter ; Hviid, Lars. / Enzyme-linked immunosorbent assay for activation of the classical complement pathway by Plasmodium falciparum-infected erythrocyte surface antigen-specific antibodies. I: Methods in Molecular Biology. 2022 ; Bind 2470. s. 673-678.

Bibtex

@article{e15962f0439142d783b822298a3fe028,

title = "Enzyme-linked immunosorbent assay for activation of the classical complement pathway by Plasmodium falciparum-infected erythrocyte surface antigen-specific antibodies",

abstract = "Enzyme-linked immunosorbent assays (ELISA) have a wide range of applications, ranging from specific antibody titer determination to quantification of any biological or non-biological substance with a specific binding partner (usually an antibody). The activity of biological cascades, such as the complement cascade of the innate immune system, can also be assessed by ELISA. We present here an assay optimized for the detection of the activation of the classical complement pathway by polyclonal and monoclonal antibodies (mAbs) specific for Plasmodium falciparum-infected erythrocyte surface antigens.",

keywords = "Antibodies, Protozoan, Antigens, Protozoan, Antigens, Surface/metabolism, Complement Pathway, Classical, Enzyme-Linked Immunosorbent Assay, Erythrocytes/metabolism, Humans, Malaria, Falciparum, Plasmodium falciparum/metabolism, Protozoan Proteins/metabolism",

author = "Larsen, {Mads Delbo} and Rafael Bayarri-Olmos and Peter Garred and Lars Hviid",

note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2022",

doi = "10.1007/978-1-0716-2189-9_50",

language = "English",

volume = "2470",

pages = "673--678",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Enzyme-linked immunosorbent assay for activation of the classical complement pathway by Plasmodium falciparum-infected erythrocyte surface antigen-specific antibodies

AU - Larsen, Mads Delbo

AU - Bayarri-Olmos, Rafael

AU - Garred, Peter

AU - Hviid, Lars

PY - 2022

Y1 - 2022

N2 - Enzyme-linked immunosorbent assays (ELISA) have a wide range of applications, ranging from specific antibody titer determination to quantification of any biological or non-biological substance with a specific binding partner (usually an antibody). The activity of biological cascades, such as the complement cascade of the innate immune system, can also be assessed by ELISA. We present here an assay optimized for the detection of the activation of the classical complement pathway by polyclonal and monoclonal antibodies (mAbs) specific for Plasmodium falciparum-infected erythrocyte surface antigens.

AB - Enzyme-linked immunosorbent assays (ELISA) have a wide range of applications, ranging from specific antibody titer determination to quantification of any biological or non-biological substance with a specific binding partner (usually an antibody). The activity of biological cascades, such as the complement cascade of the innate immune system, can also be assessed by ELISA. We present here an assay optimized for the detection of the activation of the classical complement pathway by polyclonal and monoclonal antibodies (mAbs) specific for Plasmodium falciparum-infected erythrocyte surface antigens.

KW - Antibodies, Protozoan

KW - Antigens, Protozoan

KW - Antigens, Surface/metabolism

KW - Complement Pathway, Classical

KW - Enzyme-Linked Immunosorbent Assay

KW - Erythrocytes/metabolism

KW - Humans

KW - Malaria, Falciparum

KW - Plasmodium falciparum/metabolism

KW - Protozoan Proteins/metabolism

U2 - 10.1007/978-1-0716-2189-9_50

DO - 10.1007/978-1-0716-2189-9_50

M3 - Journal article

C2 - 35881382

VL - 2470

SP - 673

EP - 678

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

ID: 315202007