Antimicrobial and Antivirulence Action of Eugenia brejoensis Essential Oil in vitro and in vivo Invertebrate Models

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Antimicrobial and Antivirulence Action of Eugenia brejoensis Essential Oil in vitro and in vivo Invertebrate Models. / Filho, Clovis Macêdo Bezerra; da Silva, Luís Cláudio Nascimento; da Silva, Márcia Vanusa; Løbner-Olesen, Anders; Struve, Carsten; Krogfelt, Karen Angeliki; Correia, Maria Tereza dos Santos; Oliva, Maria Luiza Vilela.

I: Frontiers in Microbiology, Bind 11, 424, 2020.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Filho, CMB, da Silva, LCN, da Silva, MV, Løbner-Olesen, A, Struve, C, Krogfelt, KA, Correia, MTDS & Oliva, MLV 2020, 'Antimicrobial and Antivirulence Action of Eugenia brejoensis Essential Oil in vitro and in vivo Invertebrate Models', Frontiers in Microbiology, bind 11, 424. https://doi.org/10.3389/fmicb.2020.00424

APA

Filho, C. M. B., da Silva, L. C. N., da Silva, M. V., Løbner-Olesen, A., Struve, C., Krogfelt, K. A., Correia, M. T. D. S., & Oliva, M. L. V. (2020). Antimicrobial and Antivirulence Action of Eugenia brejoensis Essential Oil in vitro and in vivo Invertebrate Models. Frontiers in Microbiology, 11, [424]. https://doi.org/10.3389/fmicb.2020.00424

Vancouver

Filho CMB, da Silva LCN, da Silva MV, Løbner-Olesen A, Struve C, Krogfelt KA o.a. Antimicrobial and Antivirulence Action of Eugenia brejoensis Essential Oil in vitro and in vivo Invertebrate Models. Frontiers in Microbiology. 2020;11. 424. https://doi.org/10.3389/fmicb.2020.00424

Author

Filho, Clovis Macêdo Bezerra ; da Silva, Luís Cláudio Nascimento ; da Silva, Márcia Vanusa ; Løbner-Olesen, Anders ; Struve, Carsten ; Krogfelt, Karen Angeliki ; Correia, Maria Tereza dos Santos ; Oliva, Maria Luiza Vilela. / Antimicrobial and Antivirulence Action of Eugenia brejoensis Essential Oil in vitro and in vivo Invertebrate Models. I: Frontiers in Microbiology. 2020 ; Bind 11.

Bibtex

@article{1a7870399f5c409babec222c8842a907,
title = "Antimicrobial and Antivirulence Action of Eugenia brejoensis Essential Oil in vitro and in vivo Invertebrate Models",
abstract = "Eugenia brejoensis L. (Myrtaceae) is an endemic plant from caatinga ecosystem (brazilian semi-arid) which have an E. brejoensis essential oil (EbEO) with reported antimicrobial activity. In this work, in vitro and in vivo models were used to characterize the inhibitory effects of EbEO in relation to Staphylococcus aureus. EbEO inhibited the growth of all tested S. aureus strains (including multidrug resistance isolates) with values ranging from 8 to 516 μg/mL. EbEO also synergistically increased the action of ampicillim, chloramphenicol, and kanamycin. The treatment with subinhibitory concentrations (Sub-MIC) of EbEO decreased S. aureus hemolytic activity and its ability to survive in human blood. EbEO strongly reduced the levels of staphyloxanthin (STX), an effect related to increased susceptibility of S. aureus to hydrogen peroxide. The efficacy of EbEO against S. aureus was further demonstrated using Caenorhabditis elegans and Galleria mellonella. EbEO increased the lifespan of both organisms infected by S. aureus, reducing the bacterial load. In addition, EbEO reduced the severity of S. aureus infection in G. mellonella, as shown by lower levels of melanin production in those larvae. In summary, our data suggest that EbEO is a potential source of lead molecules for development of new therapeutic alternatives against S. aureus.",
keywords = "Caenorhabditis elegans, Galleria melonella, infections models, multidrug resistance, natural products, Staphylococcus aureus, virulence factors",
author = "Filho, {Clovis Mac{\^e}do Bezerra} and {da Silva}, {Lu{\'i}s Cl{\'a}udio Nascimento} and {da Silva}, {M{\'a}rcia Vanusa} and Anders L{\o}bner-Olesen and Carsten Struve and Krogfelt, {Karen Angeliki} and Correia, {Maria Tereza dos Santos} and Oliva, {Maria Luiza Vilela}",
year = "2020",
doi = "10.3389/fmicb.2020.00424",
language = "English",
volume = "11",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S.A.",

}

RIS

TY - JOUR

T1 - Antimicrobial and Antivirulence Action of Eugenia brejoensis Essential Oil in vitro and in vivo Invertebrate Models

AU - Filho, Clovis Macêdo Bezerra

AU - da Silva, Luís Cláudio Nascimento

AU - da Silva, Márcia Vanusa

AU - Løbner-Olesen, Anders

AU - Struve, Carsten

AU - Krogfelt, Karen Angeliki

AU - Correia, Maria Tereza dos Santos

AU - Oliva, Maria Luiza Vilela

PY - 2020

Y1 - 2020

N2 - Eugenia brejoensis L. (Myrtaceae) is an endemic plant from caatinga ecosystem (brazilian semi-arid) which have an E. brejoensis essential oil (EbEO) with reported antimicrobial activity. In this work, in vitro and in vivo models were used to characterize the inhibitory effects of EbEO in relation to Staphylococcus aureus. EbEO inhibited the growth of all tested S. aureus strains (including multidrug resistance isolates) with values ranging from 8 to 516 μg/mL. EbEO also synergistically increased the action of ampicillim, chloramphenicol, and kanamycin. The treatment with subinhibitory concentrations (Sub-MIC) of EbEO decreased S. aureus hemolytic activity and its ability to survive in human blood. EbEO strongly reduced the levels of staphyloxanthin (STX), an effect related to increased susceptibility of S. aureus to hydrogen peroxide. The efficacy of EbEO against S. aureus was further demonstrated using Caenorhabditis elegans and Galleria mellonella. EbEO increased the lifespan of both organisms infected by S. aureus, reducing the bacterial load. In addition, EbEO reduced the severity of S. aureus infection in G. mellonella, as shown by lower levels of melanin production in those larvae. In summary, our data suggest that EbEO is a potential source of lead molecules for development of new therapeutic alternatives against S. aureus.

AB - Eugenia brejoensis L. (Myrtaceae) is an endemic plant from caatinga ecosystem (brazilian semi-arid) which have an E. brejoensis essential oil (EbEO) with reported antimicrobial activity. In this work, in vitro and in vivo models were used to characterize the inhibitory effects of EbEO in relation to Staphylococcus aureus. EbEO inhibited the growth of all tested S. aureus strains (including multidrug resistance isolates) with values ranging from 8 to 516 μg/mL. EbEO also synergistically increased the action of ampicillim, chloramphenicol, and kanamycin. The treatment with subinhibitory concentrations (Sub-MIC) of EbEO decreased S. aureus hemolytic activity and its ability to survive in human blood. EbEO strongly reduced the levels of staphyloxanthin (STX), an effect related to increased susceptibility of S. aureus to hydrogen peroxide. The efficacy of EbEO against S. aureus was further demonstrated using Caenorhabditis elegans and Galleria mellonella. EbEO increased the lifespan of both organisms infected by S. aureus, reducing the bacterial load. In addition, EbEO reduced the severity of S. aureus infection in G. mellonella, as shown by lower levels of melanin production in those larvae. In summary, our data suggest that EbEO is a potential source of lead molecules for development of new therapeutic alternatives against S. aureus.

KW - Caenorhabditis elegans

KW - Galleria melonella

KW - infections models

KW - multidrug resistance

KW - natural products

KW - Staphylococcus aureus

KW - virulence factors

U2 - 10.3389/fmicb.2020.00424

DO - 10.3389/fmicb.2020.00424

M3 - Journal article

C2 - 32265869

AN - SCOPUS:85082693950

VL - 11

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 424

ER -

ID: 239860236