Two intestinal specific nuclear factors binding to the lactase-phlorizin hydrolase and sucrase-isomaltase promoters are functionally related oligomeric molecules.
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Two intestinal specific nuclear factors binding to the lactase-phlorizin hydrolase and sucrase-isomaltase promoters are functionally related oligomeric molecules. / Troelsen, J T; Mitchelmore, C; Sjöström, H; Norén, O; Olsen, Jørgen; Hansen, Gert Helge.
I: FEBS Letters, Bind 342, Nr. 3, 1994, s. 297-301.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Two intestinal specific nuclear factors binding to the lactase-phlorizin hydrolase and sucrase-isomaltase promoters are functionally related oligomeric molecules.
AU - Troelsen, J T
AU - Mitchelmore, C
AU - Sjöström, H
AU - Norén, O
AU - Olsen, Jørgen
AU - Hansen, Gert Helge
N1 - Keywords: Base Sequence; Cell Line; DNA-Binding Proteins; Gene Expression Regulation; Glycosylceramidase; Humans; Molecular Sequence Data; Molecular Weight; Nuclear Proteins; Oligodeoxyribonucleotides; Promoter Regions (Genetics); RNA, Messenger; Sucrase-Isomaltase Complex; Transcription Factors
PY - 1994
Y1 - 1994
N2 - Lactase-phlorizin hydrolase (LPH) and sucrase-isomaltase (SI) are enterocyte-specific gene products. The identification of regulatory cis-elements in the promoter of these two genes has enabled us to carry out comparative studies of the corresponding intestinal-specific nuclear factors (NF-LPH1 and SIF1-BP). Electrophoretic mobility shift assays demonstrated that the two nuclear factors compete for binding on the same cis-elements. The molecular size of the DNA binding polypeptide is estimated to be approximately 50 kDa for both factors. In the native form the factors are found as 250 kDa oligomeric complexes. Based on these results NF-LPH1 and SIF1-BP are suggested to be either identical or closely related molecules.
AB - Lactase-phlorizin hydrolase (LPH) and sucrase-isomaltase (SI) are enterocyte-specific gene products. The identification of regulatory cis-elements in the promoter of these two genes has enabled us to carry out comparative studies of the corresponding intestinal-specific nuclear factors (NF-LPH1 and SIF1-BP). Electrophoretic mobility shift assays demonstrated that the two nuclear factors compete for binding on the same cis-elements. The molecular size of the DNA binding polypeptide is estimated to be approximately 50 kDa for both factors. In the native form the factors are found as 250 kDa oligomeric complexes. Based on these results NF-LPH1 and SIF1-BP are suggested to be either identical or closely related molecules.
M3 - Journal article
C2 - 8150088
VL - 342
SP - 297
EP - 301
JO - F E B S Letters
JF - F E B S Letters
SN - 0014-5793
IS - 3
ER -
ID: 6586676