Tissue-type plasminogen activator in somatostatin cells of rat pancreas and hypothalamus
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Tissue-type plasminogen activator in somatostatin cells of rat pancreas and hypothalamus. / Kristensen, P; Larsson, L I; Danø, K; Nielsen, Jens Høiriis.
I: Endocrinology, Bind 121, Nr. 6, 12.1987, s. 2238-44.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Tissue-type plasminogen activator in somatostatin cells of rat pancreas and hypothalamus
AU - Kristensen, P
AU - Larsson, L I
AU - Danø, K
AU - Nielsen, Jens Høiriis
PY - 1987/12
Y1 - 1987/12
N2 - Plasminogen activators (PAs) proteolytically convert plasminogen to plasmin, which, in turn, can degrade most proteins. This system has been implicated in a variety of biological processes. Using immunocytochemical methods, we here describe the localization of tissue-type PA (t-PA) in rat somatostatin cells. In the pancreatic islets, a low number of strongly t-PA-immunoreactive cells was found. By sequential staining, we found these cells to constitute a subpopulation of the somatostatin cells. Biochemical analysis of extracts from isolated rat islets demonstrated the presence of t-PA, and immunoblotting analysis demonstrated one band with a similar electrophoretic mobility. No urokinase-type PA immunoreactivity was found in the rat endocrine pancreas. A granular t-PA immunoreactivity resembling that found in adjacent sections with somatostatin antiserum was found in the median eminence of the hypothalamus. No t-PA immunoreactivity could be detected in somatostatin cells of the gastric and intestinal mucosa.
AB - Plasminogen activators (PAs) proteolytically convert plasminogen to plasmin, which, in turn, can degrade most proteins. This system has been implicated in a variety of biological processes. Using immunocytochemical methods, we here describe the localization of tissue-type PA (t-PA) in rat somatostatin cells. In the pancreatic islets, a low number of strongly t-PA-immunoreactive cells was found. By sequential staining, we found these cells to constitute a subpopulation of the somatostatin cells. Biochemical analysis of extracts from isolated rat islets demonstrated the presence of t-PA, and immunoblotting analysis demonstrated one band with a similar electrophoretic mobility. No urokinase-type PA immunoreactivity was found in the rat endocrine pancreas. A granular t-PA immunoreactivity resembling that found in adjacent sections with somatostatin antiserum was found in the median eminence of the hypothalamus. No t-PA immunoreactivity could be detected in somatostatin cells of the gastric and intestinal mucosa.
KW - Animals
KW - Hypothalamus
KW - Immunoenzyme Techniques
KW - Islets of Langerhans
KW - Male
KW - Median Eminence
KW - Molecular Weight
KW - Rats
KW - Rats, Inbred Strains
KW - Somatostatin
KW - Tissue Plasminogen Activator
M3 - Journal article
C2 - 2890516
VL - 121
SP - 2238
EP - 2244
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
SN - 0013-7227
IS - 6
ER -
ID: 47974815