The transcriptome of hand eczema assessed by tape stripping
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The transcriptome of hand eczema assessed by tape stripping. / Sølberg, Julie B.K.; Quaade, Anna S.; Jacobsen, Stine B.; Andersen, Jeppe D.; Kampmann, Marie Louise; Morling, Niels; Litman, Thomas; Thyssen, Jacob P.; Johansen, Jeanne D.
I: Contact Dermatitis, Bind 86, Nr. 2, 2022, s. 71-79.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - The transcriptome of hand eczema assessed by tape stripping
AU - Sølberg, Julie B.K.
AU - Quaade, Anna S.
AU - Jacobsen, Stine B.
AU - Andersen, Jeppe D.
AU - Kampmann, Marie Louise
AU - Morling, Niels
AU - Litman, Thomas
AU - Thyssen, Jacob P.
AU - Johansen, Jeanne D.
N1 - Publisher Copyright: © 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
PY - 2022
Y1 - 2022
N2 - Background: No biomarkers have been identified that can classify subtypes of hand eczema (HE). Although skin biopsies represent the gold standard for investigations of the skin, the invasive technique is not favorable when investigating skin from sensitive areas. Recent advances in the use of skin-tape strips for molecular investigations enable noninvasive investigations of HE. Objective: By using whole transcriptome sequencing (WTS), the molecular profile of HE according to different localizations on the hands, etiologies, and clinical/morphological subtypes was investigated. Methods: Thirty adult, Danish HE patients, 12 with and 18 without concurrent atopic dermatitis (AD), as well as 16 controls were included. Tape strip samples were collected from lesional, nonlesional, and healthy skin. Total RNA was extracted and WTS was performed. Results: The largest molecular difference of HE patients with and without AD was found in nonlesional skin areas and included a downregulation of CXCL8 for HE patients without AD. Differences between allergic and irritant contact dermatitis included promising epidermal biomarkers such as EPHA1. Conclusion: Skin tape strip samples could be used to assess the gene expression profile of HE on different localizations of the hands. The skin tape strip method identified new molecular markers that showed promising result for the identification of HE subtypes.
AB - Background: No biomarkers have been identified that can classify subtypes of hand eczema (HE). Although skin biopsies represent the gold standard for investigations of the skin, the invasive technique is not favorable when investigating skin from sensitive areas. Recent advances in the use of skin-tape strips for molecular investigations enable noninvasive investigations of HE. Objective: By using whole transcriptome sequencing (WTS), the molecular profile of HE according to different localizations on the hands, etiologies, and clinical/morphological subtypes was investigated. Methods: Thirty adult, Danish HE patients, 12 with and 18 without concurrent atopic dermatitis (AD), as well as 16 controls were included. Tape strip samples were collected from lesional, nonlesional, and healthy skin. Total RNA was extracted and WTS was performed. Results: The largest molecular difference of HE patients with and without AD was found in nonlesional skin areas and included a downregulation of CXCL8 for HE patients without AD. Differences between allergic and irritant contact dermatitis included promising epidermal biomarkers such as EPHA1. Conclusion: Skin tape strip samples could be used to assess the gene expression profile of HE on different localizations of the hands. The skin tape strip method identified new molecular markers that showed promising result for the identification of HE subtypes.
KW - atopic hand eczema
KW - contact dermatitis
KW - subtypes of hand eczema
KW - tape stripping
KW - transcriptomics
U2 - 10.1111/cod.14015
DO - 10.1111/cod.14015
M3 - Journal article
C2 - 34812515
AN - SCOPUS:85120791754
VL - 86
SP - 71
EP - 79
JO - Contact Dermatitis
JF - Contact Dermatitis
SN - 0105-1873
IS - 2
ER -
ID: 287705142