The impact of VEGF and bFGF on vascular stereomorphology in the context of angiogenic neo-arborisation after vascular induction
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
The impact of VEGF and bFGF on vascular stereomorphology in the context of angiogenic neo-arborisation after vascular induction. / Polykandriotis, E; Arkudas, A; Beier, J P; Dragu, A; Rath, S; Pryymachuk, G; Schmidt, V J; Lametschwandtner, A; Horch, R E; Kneser, U.
I: Journal of Electron Microscopy, Bind 60, Nr. 4, 2011, s. 267-74.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - The impact of VEGF and bFGF on vascular stereomorphology in the context of angiogenic neo-arborisation after vascular induction
AU - Polykandriotis, E
AU - Arkudas, A
AU - Beier, J P
AU - Dragu, A
AU - Rath, S
AU - Pryymachuk, G
AU - Schmidt, V J
AU - Lametschwandtner, A
AU - Horch, R E
AU - Kneser, U
PY - 2011
Y1 - 2011
N2 - The aim of this in vivo study was to gather quantitative information on the three-dimensional morphology of a new vascular network under the influence of angioactive growth factors. For this purpose, the arteriovenous loop model was used in 10 Lewis rats to generate a bioartificial vascular assembly by means of vascular induction. In this model, an isolated organoid is created in the medial thigh of the animal by methods of tissue engineering. A fibrin gel containing vascular endothelial growth factor (VEGF(165)) and basic fibroblastic growth factor (bFGF) was used as a matrix in the effect group (GF+). Fibrin matrices devoid of growth factors were used as controls (GF-). A microvascular replica of the organoid was created by means of corrosion casting and the network was investigated on stereo-paired images obtained by scanning electron microscopy. Vectors of intercapillary and interbranching distances as well as the diameter of the pores in the intussusceptive events diameter and the ratio of sprouting versus intussusceptive angiogenic events were compared in the two groups. The results were highly significant. In the GF+ group there were more profound three-dimensional morphological traits of angiogenesis, whereas advanced neovascularisation in the phase of remodelling was demonstrated by a higher incidence of intussusception, compared to control. These results illustrate the importance of morphological studies with focus on the generation of three-dimensional vascular networks.
AB - The aim of this in vivo study was to gather quantitative information on the three-dimensional morphology of a new vascular network under the influence of angioactive growth factors. For this purpose, the arteriovenous loop model was used in 10 Lewis rats to generate a bioartificial vascular assembly by means of vascular induction. In this model, an isolated organoid is created in the medial thigh of the animal by methods of tissue engineering. A fibrin gel containing vascular endothelial growth factor (VEGF(165)) and basic fibroblastic growth factor (bFGF) was used as a matrix in the effect group (GF+). Fibrin matrices devoid of growth factors were used as controls (GF-). A microvascular replica of the organoid was created by means of corrosion casting and the network was investigated on stereo-paired images obtained by scanning electron microscopy. Vectors of intercapillary and interbranching distances as well as the diameter of the pores in the intussusceptive events diameter and the ratio of sprouting versus intussusceptive angiogenic events were compared in the two groups. The results were highly significant. In the GF+ group there were more profound three-dimensional morphological traits of angiogenesis, whereas advanced neovascularisation in the phase of remodelling was demonstrated by a higher incidence of intussusception, compared to control. These results illustrate the importance of morphological studies with focus on the generation of three-dimensional vascular networks.
KW - Animals
KW - Arteriovenous Anastomosis/anatomy & histology
KW - Capillaries/drug effects
KW - Corrosion Casting
KW - Femoral Artery/anatomy & histology
KW - Femoral Vein/anatomy & histology
KW - Fibrin
KW - Fibroblast Growth Factor 2/metabolism
KW - Gels
KW - Imaging, Three-Dimensional
KW - Microscopy, Electron, Scanning
KW - Neovascularization, Physiologic/drug effects
KW - Organ Culture Techniques
KW - Rats
KW - Rats, Inbred Lew
KW - Regenerative Medicine
KW - Tissue Engineering/methods
KW - Vascular Endothelial Growth Factor A/metabolism
U2 - 10.1093/jmicro/dfr025
DO - 10.1093/jmicro/dfr025
M3 - Journal article
C2 - 21622976
VL - 60
SP - 267
EP - 274
JO - Microscopy (Oxford, England)
JF - Microscopy (Oxford, England)
SN - 2050-5698
IS - 4
ER -
ID: 329569275