The Bacillus cereus Strain EC9 Primes the Plant Immune System for Superior Biocontrol of Fusarium oxysporum
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The Bacillus cereus Strain EC9 Primes the Plant Immune System for Superior Biocontrol of Fusarium oxysporum. / Madriz-Ordeñana, Kenneth; Pazarlar, Sercan; Jørgensen, Hans Jørgen Lyngs; Nielsen, Tue Kjærgaard; Zhang, Yingqi; Nielsen, Kai Lønne; Hansen, Lars Hestbjerg; Thordal-Christensen, Hans.
I: Plants, Bind 11, Nr. 5, 687, 2022.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - The Bacillus cereus Strain EC9 Primes the Plant Immune System for Superior Biocontrol of Fusarium oxysporum
AU - Madriz-Ordeñana, Kenneth
AU - Pazarlar, Sercan
AU - Jørgensen, Hans Jørgen Lyngs
AU - Nielsen, Tue Kjærgaard
AU - Zhang, Yingqi
AU - Nielsen, Kai Lønne
AU - Hansen, Lars Hestbjerg
AU - Thordal-Christensen, Hans
N1 - Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022
Y1 - 2022
N2 - Antibiosis is a key feature widely exploited to develop biofungicides based on the ability of biological control agents (BCAs) to produce fungitoxic compounds. A less recognised attribute of plant-associated beneficial microorganisms is their ability to stimulate the plant immune system, which may provide long-term, systemic self-protection against different types of pathogens. By using conventional antifungal in vitro screening coupled with in planta assays, we found antifungal and non-antifungal Bacillus strains that protected the ornamental plant Kalanchoe against the soil-borne pathogen Fusarium oxysporum in experimental and commercial production settings. Further examination of one antifungal and one non-antifungal strain indicated that high protection efficacy in planta did not correlate with antifungal activity in vitro. Whole-genome sequencing showed that the non-antifungal strain EC9 lacked the biosynthetic gene clusters associated with typical antimi-crobial compounds. Instead, this bacterium triggers the expression of marker genes for the jasmonic and salicylic acid defence pathways, but only after pathogen challenge, indicating that this strain may protect Kalanchoe plants by priming immunity. We suggest that the stimulation of the plant immune system is a promising mode of action of BCAs for the development of novel biological crop protection products.
AB - Antibiosis is a key feature widely exploited to develop biofungicides based on the ability of biological control agents (BCAs) to produce fungitoxic compounds. A less recognised attribute of plant-associated beneficial microorganisms is their ability to stimulate the plant immune system, which may provide long-term, systemic self-protection against different types of pathogens. By using conventional antifungal in vitro screening coupled with in planta assays, we found antifungal and non-antifungal Bacillus strains that protected the ornamental plant Kalanchoe against the soil-borne pathogen Fusarium oxysporum in experimental and commercial production settings. Further examination of one antifungal and one non-antifungal strain indicated that high protection efficacy in planta did not correlate with antifungal activity in vitro. Whole-genome sequencing showed that the non-antifungal strain EC9 lacked the biosynthetic gene clusters associated with typical antimi-crobial compounds. Instead, this bacterium triggers the expression of marker genes for the jasmonic and salicylic acid defence pathways, but only after pathogen challenge, indicating that this strain may protect Kalanchoe plants by priming immunity. We suggest that the stimulation of the plant immune system is a promising mode of action of BCAs for the development of novel biological crop protection products.
KW - Antimicrobial secondary metabolites
KW - Biological control agents
KW - Defence priming
KW - Induced resistance
KW - Kalanchoe blossfeldiana
U2 - 10.3390/plants11050687
DO - 10.3390/plants11050687
M3 - Journal article
C2 - 35270157
AN - SCOPUS:85126069391
VL - 11
JO - Plants
JF - Plants
SN - 2223-7747
IS - 5
M1 - 687
ER -
ID: 300917220