The ability to form full-length intron RNA circles is a general property of nuclear group I introns
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The ability to form full-length intron RNA circles is a general property of nuclear group I introns. / Nielsen, Henrik; Fiskaa, Tonje; Birgisdottir, Asa Birna; Haugen, Peik; Einvik, Christer; Johansen, Steinar.
I: RNA: A publication of the RNA Society, Bind 9, Nr. 12, 2003, s. 1464-75.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - The ability to form full-length intron RNA circles is a general property of nuclear group I introns
AU - Nielsen, Henrik
AU - Fiskaa, Tonje
AU - Birgisdottir, Asa Birna
AU - Haugen, Peik
AU - Einvik, Christer
AU - Johansen, Steinar
N1 - Keywords: Base Sequence; Cell Nucleus; DNA Primers; Exons; Hydrolysis; Introns; Molecular Sequence Data; Mutagenesis; Nucleic Acid Conformation; RNA; RNA Precursors; RNA Splicing
PY - 2003
Y1 - 2003
N2 - In addition to splicing, group I intron RNA is capable of an alternative two-step processing pathway that results in the formation of full-length intron circular RNA. The circularization pathway is initiated by hydrolytic cleavage at the 3' splice site and followed by a transesterification reaction in which the intron terminal guanosine attacks the 5' splice site presented in a structure analogous to that of the first step of splicing. The products of the reactions are full-length circular intron and unligated exons. For this reason, the circularization reaction is to the benefit of the intron at the expense of the host. The circularization pathway has distinct structural requirements that differ from those of splicing and appears to be specifically suppressed in vivo. The ability to form full-length circles is found in all types of nuclear group I introns, including those from the Tetrahymena ribosomal DNA. The biological function of the full-length circles is not known, but the fact that the circles contain the entire genetic information of the intron suggests a role in intron mobility.
AB - In addition to splicing, group I intron RNA is capable of an alternative two-step processing pathway that results in the formation of full-length intron circular RNA. The circularization pathway is initiated by hydrolytic cleavage at the 3' splice site and followed by a transesterification reaction in which the intron terminal guanosine attacks the 5' splice site presented in a structure analogous to that of the first step of splicing. The products of the reactions are full-length circular intron and unligated exons. For this reason, the circularization reaction is to the benefit of the intron at the expense of the host. The circularization pathway has distinct structural requirements that differ from those of splicing and appears to be specifically suppressed in vivo. The ability to form full-length circles is found in all types of nuclear group I introns, including those from the Tetrahymena ribosomal DNA. The biological function of the full-length circles is not known, but the fact that the circles contain the entire genetic information of the intron suggests a role in intron mobility.
M3 - Journal article
C2 - 14624003
VL - 9
SP - 1464
EP - 1475
JO - RNA
JF - RNA
SN - 1355-8382
IS - 12
ER -
ID: 14612055